Formation of free hydroxyl radicals after pentylenetetrazol-induced seizure and kindling
Formation of free hydroxyl radicals after pentylenetetrazol-induced seizure and kindling
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DOI：
10.1016/s0006-8993(99)02084-3
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发表时间：
1999-11-20
期刊：
影响因子：
2.9
通讯作者：
Jantze, H
中科院分区：
文献类型：
--
作者：
Rauca, C;Zerbe, R;Jantze, H
本研究表明，自由基已牵连戊四氮（PTZ）诱导的癫痫发作和点燃。在我们的实验中，我们使用了一种方法，其中自由羟基自由基（。OH）在体内被全身应用的水杨酸盐捕获，产生稳定且可定量的产物2，3-二羟基苯甲酸（DHBA）和2，5-DHBA。我们研究了2，3-和2，5-DHBA在用48 mg/kg PTZ的急性惊厥诱导剂量处理的大鼠的全脑（不包括小脑）中的形成，以及在最后一次PTZ注射后的不同时间注射点燃剂量37.5 mg/kg PTZ的PTZ点燃动物中的形成。与急性惊厥动物相比，在水杨酸盐治疗后1分钟进行PTZ应用时，在点燃大鼠中观察到OH的增加。与用NaCl而不是PTZ治疗的动物相比，在应用PTZ后30分钟，两个惊厥组中的DHBA的量显著增加。PTZ后60分钟，DHBA水平标准化为NaCl对照值。(C)1999 Elsevier Science B. V.保留所有权利。
The present study indicates that free radicals have been implicated in pentylenetetrazol (PTZ)-induced seizure and kindling. In our experiments we used a method in which free hydroxyl radicals (. OH) are trapped by systemically applied salicylate in vivo, resulting in the stable and quantifiable products, 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA. We investigated the formation of both 2,3- and 2,5-DHBA in the whole brain (without cerebellum) of rats treated with an acute seizure-inducing dose of 48 mg/kg PTZ and in PTZ-kindled animals which were injected with the kindling dose of 37.5 mg/kg PTZ at different times after the last PTZ injection. An increase of OH was observed in kindled rats compared with acutely convulsing animals when the PTZ application was performed 1 min after the salicylate treatment. The amount of DHBAs increased significantly in both convulsed groups 30 min after the application of PTZ compared with animals treated with NaCl instead of PTZ. Sixty minutes after PTZ the DHBA levels normalized to NaCl control values. (C) 1999 Elsevier Science B.V. All rights reserved.