Proteomic analysis of rice leaf sheath during drought stress
Proteomic analysis of rice leaf sheath during drought stress
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DOI：
10.1021/pr050291g
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Komatsu, S
中科院分区：
文献类型：
--
作者：
Ali, GM;Komatsu, S
干旱是限制雨养低地和高地生产力的最严重因素之一。为了研究水稻对干旱胁迫的初始反应，利用蛋白质组学方法分析了蛋白质表达的变化。以2周龄的水稻幼苗为材料，在干旱条件下处理2 ~ 6 d，提取叶鞘中的蛋白质，经双向聚丙烯酰胺凝胶电泳分离，考马斯亮蓝染色。干旱胁迫2 ~ 6d后，10种蛋白质含量增加，2种蛋白质含量下降。这些蛋白质的功能类别被确定为防御，能量，代谢，细胞结构和信号转导。除了干旱胁迫外，还分析了几种不同胁迫条件下蛋白质的积累。肌动蛋白解聚因子，捕光复合物链11，超氧化物歧化酶和盐诱导蛋白的水平发生变化的干旱和渗透胁迫，但不冷或盐胁迫，或脱落酸处理。分析了干旱胁迫对耐旱水稻品种叶鞘蛋白质含量的影响。光捕获复合物链11和肌动蛋白解聚因子存在于高水平的耐旱水稻品种胁迫前的应用。干旱胁迫下，肌动蛋白解聚因子在叶片、叶鞘和根中表达。这些结果表明，肌动蛋白解聚因子是干旱胁迫诱导的靶蛋白之一。
Drought is one of the most severe limitations on the productivity of rainfed lowland and upland rice. To investigate the initial response of rice to drought stress, changes in protein expression were analyzed using a proteomic approach. Two-week-old rice seedlings were exposed to drought conditions from 2 to 6 days, and proteins were extracted from leaf sheaths, separated by two-dimensional polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. After drought stress for 2 to 6 days, 10 proteins increased in abundance and the level of 2 proteins decreased. The functional categories of these proteins were identified as defense, energy, metabolism, cell structure, and signal transduction. In addition to drought stress, accumulations of protein were analyzed under several different stress conditions. The levels of an actin depolymerizing factor, a light harvesting complex chain 11, a superoxidase dismutase and a salt-induced protein were changed by drought and osmotic stresses, but not cold or salt stresses, or abscisic acid treatment. The effect of drought stress on protein in the leaf sheaths of drought-tolerant rice cultivar was also analyzed. The light harvesting complex chain 11 and the actin depolymerizing factor were present at high levels in a drought-tolerant rice cultivar before stress application. With drought stress, actin depolymerizing factor was expressed in leaf blades, leaf sheaths, and roots. These results suggest that actin depolymerizing factor is one of the target proteins induced by drought stress.