ASSIGNMENT OF PARAMAGNETICALLY SHIFTED RESONANCES IN THE H-1-NMR SPECTRUM OF HORSE FERRICYTOCHROME-C
ASSIGNMENT OF PARAMAGNETICALLY SHIFTED RESONANCES IN THE H-1-NMR SPECTRUM OF HORSE FERRICYTOCHROME-C
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DOI：
10.1016/s0006-3495(90)82502-9
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发表时间：
1990-01-01
影响因子：
3.4
通讯作者：
ENGLANDER, SW
中科院分区：
文献类型：
--
作者：
FENG, YQ;RODER, H;ENGLANDER, SW
用一维和二维核Overhauser和磁化转移方法研究了马铁细胞色素c的H核磁共振谱中血红素、轴向配体和其他超精细位移共振的质子共振。在铁和ferricytochrome C的混合物中的饱和转移实验的条件进行了优化，在两个氧化态的相应的共振交叉分配。获得了两个硫醚桥的次甲基质子的新的共振归属，和γ内消旋质子、丙酸六个血红素取代基、His-18的N π H和Tyr-67 OH。此外，最近报告的几项任务也得到了确认。所有的解决超精细位移共振的光谱铁细胞色素c现在确定。的费米接触所经历的血红素和配体质子的位移进行了讨论。
The proton resonances of the heme, the axial ligands, and other hyperfine-shifted resonances in the H nuclear magnetic resonance spectrum of horse ferricytochrome c have been investigated by means of one- and two-dimensional nuclear Overhauser and magnetization transfer methods. Conditions for saturation transfer experiments in mixtures of ferro- and ferricytochrome c were optimized for the cross assignment of corresponding resonances in the two oxidation states. New resonance assignments were obtained for the methine protons of both thioether bridges, the .beta. and .gamma. meso protons, the propionate six heme substituents, the N.pi.H of His-18, and the Tyr-67 OH. In addition, several recently reported assignments were confirmed. All of the resolved hyperfine-shifted resonances in the spectrum of ferricytochrome c are now identified. The Fermi contacts shifts experienced by the heme and ligand protons are discussed.