Differential segregation patterns of sperm mitochondria in embryos of the blue mussel (Mytilus edulis)
Differential segregation patterns of sperm mitochondria in embryos of the blue mussel (Mytilus edulis)
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DOI：
10.1534/genetics.166.2.883
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发表时间：
2004-02-01
期刊：
影响因子：
3.3
通讯作者：
Zouros, E
中科院分区：
文献类型：
--
作者：
Cao, LQ;Kenchington, E;Zouros, E
在贻贝中，雌性主要携带母本线粒体 DNA (mtDNA)，而雄性则在其体细胞组织中携带母本 mtDNA，在性腺中携带父本 mtDNA。这种现象被称为线粒体DNA的双单亲遗传（DUI），它与细胞器基因组的单亲遗传有很大不同。来自仅产生女儿的雌性和主要产生儿子的雌性的贻贝卵与用 MitoTracker Green FM 染色的精子受精，从而可以通过落射荧光和共聚焦显微镜观察胚胎中的精子线粒体。在只产生女儿的雌性胚胎中，精子线粒体随机分散在卵裂球中。在主要产生儿子的雌性胚胎中，精子线粒体倾向于聚集并最终形成二细胞和四细胞阶段的一个卵裂球。我们假设最终是聚合。最终出现在第一个生殖细胞中，从而解释了男性性腺中存在父本线粒体DNA的原因。这是精子线粒体在发育胚胎中不同行为的第一个证据，这可能解释了酒驾物种中性别与父本线粒体 DNA 遗传之间的紧密联系。
In Mytilus, females carry predominantly maternal mitochondrial DNA (mtDNA) but males carry maternal mtDNA in their somatic tissues and paternal mtDNA in their gonads. This phenomenon, known as doubly uniparental inheritance (DUI) of mtDNA, presents a major departure from the uniparental transmission of organelle genomes. Eggs of Mytilus edulis from females that produce exclusively daughters and from females that produce mostly sons were fertilized with sperm stained with MitoTracker Green FM, allowing observation of sperm mitochondria in the embryo by epifluorescent and confocal microscopy. In embryos from females that produce only daughters, sperm mitochondria are randomly dispersed among blastomeres. In embryos from females that produce mostly sons, sperm mitochondria tend to aggregate and end up in one blastomere in the two- and four-cell stages. We postulate that the aggregate eventually. ends up in the first germ cells, thus accounting for the presence of paternal mtDNA in the male gonad. This is the first evidence for different behaviors of sperm mitochondria in developing embryos that may explain the tight linkage between gender and inheritance of paternal mitochondrial DNA in species with DUI.