Genetic mapping and isolation of two arc3 alleles in Arabidopsis
Genetic mapping and isolation of two arc3 alleles in Arabidopsis
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拟南芥中两个 arc3 等位基因的遗传图谱和分离
DOI：
10.1007/s00299-012-1352-7
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发表时间：
2013-01-01
影响因子：
6.2
通讯作者：
Gao, Hongbo
中科院分区：
文献类型：
--
作者：
Pan, Deng;Shi, Yuhong;Gao, Hongbo
关键信息：拟南芥arc 3的两个新等位基因arc 3 -4和arc 3 -5在哥伦比亚-0生态型中被分离。使用显微镜和分子技术的突变体进行了详细的表征。叶绿体是植物细胞进行光合作用的重要细胞器。叶绿体的分裂由内部分裂机制（主要是微管蛋白样FtsZ环）和外部分裂机制（主要是动力蛋白样ARC 5环）协调。叶绿体3（ARC 3）的积累和复制对于叶绿体分裂机器的正确定位是重要的。在进化过程中，ARC 3可能取代了minicellC（MinC），这是一个参与细菌分裂位点定位的重要因素。然而，ARC 3的工作机制仍不清楚。利用正向遗传学方法，我们在拟南芥中分离到两个新的arc 3等位基因arc 3 -4和arc 3 -5，其中突变位点不同于先前报道的arc 3突变体。显微镜分析显示了更详细的，一些新的，arc 3突变体的表型。逆转录聚合酶链反应（RT-PCR）和实时定量RT-PCR（qRT-PCR）结果表明，ARC 3基因在arc 3 -4和arc 3 -5突变体中的表达不稳定。此外，预测ARC 3基因的RNA二级结构在这两个ARC 3突变体和野生型之间不同。我们的研究增加了我们对ARC 3在叶绿体分裂中的功能的理解。
KEY MESSAGE : Two new alleles of arc3 in Arabidopsis thaliana, arc3-4 and arc3-5, were isolated in the Columbia-0 ecotype. The mutants were characterized in detail using microscopy and molecular techniques. Chloroplasts are essential organelles for photosynthesis in plant cells. Division of chloroplasts is coordinated by the internal division machinery (mainly the tubulin-like FtsZ ring) and the external division machinery (mainly the dynamin-like ARC5 ring). Accumulation and replication of chloroplasts3 (ARC3) is important for the correct positioning of chloroplast division machinery. During evolution, ARC3 has probably replaced minicellC (MinC), an important factor involved in positioning of the division site in bacteria. However, the working mechanism of ARC3 is still unclear. Using forward genetic approaches, we isolated two new alleles of arc3 in Arabidopsis thaliana, arc3-4 and arc3-5, in which mutant loci differed from those of previously reported arc3 mutants. Microscopy analyses showed more detailed, and some new, phenotypes of arc3 mutants. Reverse-transcription polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR (qRT-PCR) results indicated that the mRNA of the ARC3 gene was unstable in arc3-4 and arc3-5 mutant plants. Also, RNA secondary structures of the ARC3 gene were predicted to differ between these two arc3 mutants and wild type. Our studies increase our understanding of the function of ARC3 in chloroplast division.