Content-dependent activity of lung surfactant protein B in mixtures with lipids.
Content-dependent activity of lung surfactant protein B in mixtures with lipids.
复制标题
肺表面活性蛋白 B 在与脂质的混合物中的含量依赖性活性。
DOI：
10.1152/ajplung.00431.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Notter,RH
中科院分区：
文献类型：
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作者：
Wang,Z;Baatz,JE;Holm,BA;Notter,RH
研究了表面活性蛋白（SP）-B与二棕榈酰磷脂酰胆碱（DPPC）、合成脂质（SL）和来自小牛肺表面活性物质提取物（CLSE）的纯化磷脂（PPL）的混合物的含量依赖性活性。在固定SP-B含量下，PPL/SP-B的吸附量和动态表面张力降低的顺序为PPL/SP-B> SL/SP-B > DPPC/SP-B。当SP-B含量从0.05重量%逐渐增加到0.75重量%时，所有混合物在具有增加的表面活性方面是相似的。SP-B对界面性能的影响很小，但可测量，即使在非常低的水平≤0.1%（重量）。PPL/SP-B（0.75%）具有最高的吸附和动态表面活性，接近CLSE的行为。在低磷脂浓度（1 mg/ml）下的脉动气泡研究中，所有含有0.75% SP-B的混合物达到<1 mN/m的最小表面张力。PPL或SL与SP-B（0.5%）的混合物在lmg/ml下也具有<lmN/m的最小表面张力，而DPPC/SP-B（0.5%）在2.5mg/ml下达到<lmN/m。生理活性也强烈依赖于SP-B含量。灌注SL/SP-B混合物改善切除灌洗大鼠肺中表面活性剂缺乏的压力-体积力学的能力随着SP-B含量从0.1重量%升高至0.75重量%而增加。这项研究强调了SP-B在肺表面活性剂中的关键功能活性。用于治疗呼吸道疾病的外源性表面活性剂之间SP-B含量的显著差异可能与实质性活性变化有关。
The content-dependent activity of surfactant protein (SP)-B was studied in mixtures with dipalmitoyl phosphatidylcholine (DPPC), synthetic lipids (SL), and purified phospholipids (PPL) from calf lung surfactant extract (CLSE). At fixed SP-B content, adsorption and dynamic surface tension lowering were ordered as PPL/SP-B ≈ SL/SP-B > DPPC/SP-B. All mixtures were similar in having increased surface activity as SP-B content was incrementally raised from 0.05 to 0.75% by weight. SP-B had small but measurable effects on interfacial properties even at very low levels ≤0.1% by weight. PPL/SP-B (0.75%) had the highest adsorption and dynamic surface activity, approaching the behavior of CLSE. All mixtures containing 0.75% SP-B reached minimum surface tensions <1 mN/m in pulsating bubble studies at low phospholipid concentration (1 mg/ml). Mixtures of PPL or SL with SP-B (0.5%) also had minimum surface tensions <1 mN/m at 1 mg/ml, whereas DPPC/SP-B (0.5%) reached <1 mN/m at 2.5 mg/ml. Physiological activity also was strongly dependent on SP-B content. The ability of instilled SL/SP-B mixtures to improve surfactant-deficient pressure-volume mechanics in excised lavaged rat lungs increased as SP-B content was raised from 0.1 to 0.75% by weight. This study emphasizes the crucial functional activity of SP-B in lung surfactants. Significant differences in SP-B content between exogenous surfactants used to treat respiratory disease could be associated with substantial activity variations.