Time course of leukocyte adhesion to endothelium in ischemia-reperfusion.
Time course of leukocyte adhesion to endothelium in ischemia-reperfusion.
复制标题
缺血再灌注时白细胞粘附到内皮的时间过程。
DOI：
10.1006/jsre.1996.0094
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Durán,WN
中科院分区：
文献类型：
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作者：
Milazzo,VJ;Ferrante,RJ;Sabido,F;SilvaJr,MB;Hobson2nd,RW;Durán,WN
白细胞(L)与微血管内皮(E)的粘附是L - E相互作用导致缺血再灌注时组织损伤的必要步骤。为了评估旨在改善这一必要步骤的负面影响的最佳治疗时间，我们在我们的I-R损伤模型中使用2小时缺血和1小时再灌注来研究仓鼠颊袋中L-E粘附的时间过程。[j] .中国生物医学工程学报，2006,31(2):387 - 391。用吖啶橙标记后，计数白细胞粘附（静止≥30秒）于毛细血管后小静脉（直径15-30 μm）。诱导缺血前，各组研究区域的粘附白细胞数无显著差异（1.9±0.6 vs 2.0±0.3；平均白细胞数/100-μm血管长度±SD）。再灌注10 min和20 min后，缺血区白细胞粘附与对照组比较差异无统计学意义（分别为2.7±0.5 vs 2.8±0.8,5.3±2.8 vs 2.4±0.6）。再灌注30 min后，缺血后区白细胞粘附明显增加，再灌注1 h时，缺血后区白细胞粘附较非缺血对照区升高（分别为7.8±1.3 vs 3.6±0.6,8.3±0.8 vs 4.1±0.6，P< 0.01）。再灌注30min后缺血后区域的白细胞粘附与再灌注1hr时的粘附无显著差异。这些数据表明：(1)白细胞粘附峰值发生在正常再灌注30分钟后；(2)在这一早期阶段实施化疗干预可能最有益。
Adhesion of leukocytes (L) to microvascular endothelium (E) is a required step in the L–E interaction leading to tissue injury in ischemia–reperfusion. To assess the optimum period for therapy aimed at ameliorating negative effects of this required step, we investigated the time course of L–E adhesion in the hamster cheek pouch using 2 hr of ischemia and 1 hr of reperfusion in our model of I–R injury (Am. J. Physiol.261: 1626, 1991). Leukocytes adhering (stationary for ≥30 sec) to postcapillary venules (15–30 μm in diameter) were counted after labeling with acridine orange. Prior to the induction of ischemia, there were no significant differences in the number of adherent leukocytes in each area chosen for study (1.9 ± 0.6 vs 2.0 ± 0.3; mean number of leukocytes/100-μm vessel length ± SD). After 10 and 20 min of reperfusion there was no significant difference in leukocyte adhesion in the ischemic area relative to the control (2.7 ± 0.5 vs 2.8 ± 0.8, and 5.3 ± 2.8 vs 2.4 ± 0.6, respectively). Leukocyte adherence increased significantly after 30 min of reperfusion and remained elevated at 1 hr of reperfusion in the postischemic area relative to the nonischemic control area (7.8 ± 1.3 vs 3.6 ± 0.6, and 8.3 ± 0.8 vs 4.1 ± 0.6, respectively;P< 0.01). Leukocyte adhesion in the postischemic area after 30 min reperfusion was not significantly different from the adhesion at the end of 1 hr reperfusion. These data suggest that (1) peak leukocyte adhesion occurs after 30 min of normal reperfusion and (2) postischemic therapeutic intervention may be most beneficial when instituted within this early time period.