Wolbachia supplement biotin and riboflavin to enhance reproduction in planthoppers
Wolbachia supplement biotin and riboflavin to enhance reproduction in planthoppers
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DOI：
10.1038/s41396-019-0559-9
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发表时间：
2020-03-01
期刊：
影响因子：
11
通讯作者：
Hong, Xiao-Yue
中科院分区：
文献类型：
--
作者：
Ju, Jia-Fei;Bing, Xiao-Li;Hong, Xiao-Yue
共生体介导的营养互惠作用有助于昆虫的寄主健康，特别是对于那些完全以营养不平衡的饮食为食的昆虫。在这里，我们阐明了B类维生素在内共生细菌Wolbachia与两种以植物汁液为食的昆虫--褐飞虱Laodelphax striatellus(Fall)和褐飞虱Nilparvata luens(Stal)--相互作用中的重要性。两种受感染的稻飞虱比未受感染的稻飞虱产卵更多，而将沃尔巴克氏菌转移到一种稻飞虱的未受感染的品系中，挽救了这种繁殖力不足。基因组分析表明，两种飞虱的沃尔巴克氏菌株都编码生物素和核黄素的完整生物合成操纵子，而代谢分析表明，两种飞虱体内生物素和核黄素的滴度都较高。此外，试验性添加生物素和核黄素混合物的食物恢复了未感染沃尔巴克氏菌的稻飞虱的繁殖力缺陷。此外，比较基因组分析表明，核黄素合成基因在沃尔巴克氏菌超类群中是保守的。生物素操纵子在沃尔巴克氏菌中很少见，这些操纵子有一个最近的祖先，可能是从Carddium细菌水平转移过来的。我们的研究证明了一种互惠关系，涉及沃尔巴克氏杆菌和以植物汁液为食的昆虫之间的兼性相互作用，涉及维生素B。
Symbiont-mediated nutritional mutualisms can contribute to the host fitness of insects, especially for those that feed exclusively on nutritionally unbalanced diets. Here, we elucidate the importance of B group vitamins in the association of endosymbiotic bacteria Wolbachia with two plant-sap feeding insects, the small brown planthopper, Laodelphax striatellus (Fallen), and the brown planthopper, Nilaparvata lugens (Stal). Infected planthoppers of both species laid more eggs than uninfected planthoppers, while the experimental transfer of Wolbachia into uninfected lines of one planthopper species rescued this fecundity deficit. The genomic analysis showed that Wolbachia strains from the two planthopper species encoded complete biosynthesis operons for biotin and riboflavin, while a metabolic analysis revealed that Wolbachia-infected planthoppers of both species had higher titers of biotin and riboflavin. Furthermore, experimental supplementation of food with a mixture of biotin and riboflavin recovered the fecundity deficit of Wolbachia-uninfected planthoppers. In addition, comparative genomic analysis suggested that the riboflavin synthesis genes are conserved among Wolbachia supergroups. Biotin operons are rare in Wolbachia, and those described share a recent ancestor that may have been horizontally transferred from Cardinium bacteria. Our research demonstrates a type of mutualism that involves a facultative interaction between Wolbachia and plant-sap feeding insects involving vitamin Bs.