Controlled delivery of inductive proteins, plasmid DNA and cells from tissue engineering matrices
Controlled delivery of inductive proteins, plasmid DNA and cells from tissue engineering matrices
复制标题
DOI：
10.1111/j.1600-0765.1999.tb02275.x
复制
发表时间：
1999-10-01
影响因子：
3.5
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Murphy, WL;Mooney, DJ
据估计，美国年度医疗保健预算的一半用于患有组织损失和晚期器官衰竭的患者。传统疗法固有的严重局限性需要新的组织和器官替代策略。本文讨论了用于导电、感应和基于细胞的组织替代策略的生物材料的开发。可生物降解的聚合物支架可用作组织发育的空间填充基质和组织传导方法中上皮细胞迁移的障碍。诱导方法涉及持续递送生物活性因子，例如蛋白质生长因子和 DNA，以改变局部区域的细胞功能。因子可以从高度多孔的聚合物支架中释放，从而使因子递送和组织发育同时发生。基于细胞的方法涉及在体外将细胞接种到聚合物支架上以及随后移植支架。正在开发新的支架材料，以满足特定的组织工程设计要求，并在某些情况下尝试模仿天然细胞外基质。这些策略共同提供了可预测地形成特定组织结构的可能性，并可能为牙周韧带脱离、牙槽骨吸收和分叉缺陷等问题提供解决方案。
It has been estimated that half the annual health care budget in the United States is spent on patients suffering from tissue loss and late stage organ failure. Critical limitations inherent in traditional therapies call for novel tissue and organ replacement strategies. This paper discusses development of biomaterials for conductive, inductive and cell-based tissue replacement strategies. Biodegradable polymer scaffolds can be used as space-filling matrices for tissue development and barriers to migration of epithelial cells in tissue conductive approaches. Inductive approaches involve sustained delivery of bioactive factors, such as protein growth factors and DNA, to alter cell function in localized regions. Factors can be released from highly porous polymer scaffolds to allow factor delivery and tissue development to occur in concert. Cell-based approaches involve seeding of cells onto polymeric scaffolds in vitro and subsequent transplantation of the scaffold. New scaffold materials are being developed that address specific tissue engineering design requirements, and in some cases attempt to mimic natural extracellular matrices. These strategies together offer the possibility of predictably forming specific tissue structures, and may provide solutions to problems such as periodontal ligament detachment, alveolar bone resorption and furcation defects.