Effects of paraventricular nucleus injection of CCK-8 on plasma CCK-8 levels in rats
Effects of paraventricular nucleus injection of CCK-8 on plasma CCK-8 levels in rats
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DOI：
10.1016/s0006-8993(99)02478-6
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发表时间：
2000-03-31
期刊：
影响因子：
2.9
通讯作者：
Reidelberger, RD
中科院分区：
文献类型：
--
作者：
Blevins, JE;Hamel, FG;Reidelberger, RD
本研究的目的是确定室旁核（PVN）注射500 pmol剂量的胆囊收缩素（CCK）-8是否能增加血浆CCK-8水平，足以抑制外周机制的摄食。大鼠接受单独或与H-3-标记的丙酰化CCK-8（H-3-pCCK-8）一起的CCK-8的PVN注射，并在注射后3至120分钟的不同时间采集血浆样品。血浆CCK-8水平通过测量总血浆CCK样免疫反应性（CCK-LI）和H-3-pCCK-8活性来估计。PVN注射CCK-8和3 H-pCCK-8分别产生血浆CCK-8的估计峰值增加15 +/- 11和22 +/- 3 pM。静脉输注CCK-8剂量（0.2和1 nmol/kg/h），包括抑制进食的阈值剂量，使血浆CCK-LI分别从基础水平6 +/- 1增加到49 +/- 10和166 +/- 36 pM。静脉注射600和4800 pmol CCK-8不抑制摄食。这些结果表明，PVN注射约500 pmol剂量的CCK-8不会增加血浆CCK-8水平，足以通过外周机制抑制摄食。(C)2000 Elsevier Science B. V.保留所有权利。
The aim of the study was to determine whether paraventricular nucleus (PVN) injection of an anorexic 500-pmol dose of cholecystokinin (CCK)-8 could increase plasma CCK-8 levels sufficiently to suppress feeding by a peripheral mechanism. Rats received PVN injections of CCK-8 either alone or with H-3-labelled propionylated CCK-8 (H-3-pCCK-8) and plasma samples were taken at various times from 3 to 120 min post-injection. Plasma CCK-8 levels were estimated from measurements of both total plasma CCK-like immunoreactivity (CCK-LI) and H-3-pCCK-8 activity. PVN injections of CCK-8 and 3H-pCCK-8 produced estimated peak increases in plasma CCK-8 of 15 +/- 11 and 22 +/- 3 pM, respectively. The i.v. infusion of CCK-8 doses (0.2 and 1 nmol/kg h) that bracketed the threshold dose for suppression of feeding, increased plasma CCK-LI from a basal level of 6 +/- 1 to 49 +/- 10 and 166 +/- 36 pM, respectively. The i.v. injections of 600 and 4800 pmol of CCK-8 did not suppress feeding. These results suggest that PVN injection of an anorexic 500-pmol dose of CCK-8 does not increase plasma CCK-8 levels sufficiently to suppress feeding by a peripheral mechanism. (C) 2000 Elsevier Science B.V. All rights reserved.