Cytokine expression in normal and inflamed esophageal mucosa: A study into the pathogenesis of allergic eosinophilic esophagitis
Cytokine expression in normal and inflamed esophageal mucosa: A study into the pathogenesis of allergic eosinophilic esophagitis
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DOI：
10.1097/01.mpg.0000188740.38757.d2
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发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
HogenEsch, T
中科院分区：
文献类型：
--
作者：
Gupta, SK;Fitzgerald, JF;HogenEsch, T
目的：为加深对过敏性嗜酸性食管炎(AEE)病理生理机制的认识，我们研究了正常儿童和炎症儿童食道粘膜中细胞因子和炎症细胞的表达。方法：研究对象为1~18岁的正常儿童和AEE患者(>；=15个嗜酸性粒细胞/食道粘膜活检高倍视野)。结果：研究对象为8名对照组(4名男性，平均年龄9.99岁)和11名AEE患者(8名男性，平均年龄7.15岁)。检测干扰素-γ、白介素4、白介素5、白介素13、嗜酸性粒细胞趋化因子-1、嗜酸性粒细胞趋化因子-2、嗜酸性粒细胞趋化因子-3和RANTES的基因表达。AEE患者的干扰素-γ和IL-5的表达较对照组显著上调。AEE患者IL-4和IL-13的表达与对照组相似。嗜酸性粒细胞趋化因子-1的表达在AEE患者组显著上调，而在对照组则显著上调。RANTES和eoaxin-3在两组间的表达相似。结论：AEE患者肥大细胞主要是IL-5选择性TH2反应，可能含有TH1成分，嗜酸性趋化因子可能起不同的作用。肥大细胞在AEE发病机制中的作用有待进一步研究。
Objectives: We studied the expression of cytokines and inflammatory cells in normal and inflamed esophageal mucosa of children with the aim of furthering our understanding of the pathophysiology of allergic eosinophilic esophagitis (AEE).Methods: Controls and AEE patients (>= 15 eosinophils/high-power field on esophageal mucosal biopsies) between the ages of 1 and 18 years were recruited. Esophageal biopsies were obtained for histologic examination, immunohistochemical studies, and cytokine analysis.Results: Eight controls (4 males; mean age 9.99 years) and 11 AEE patients (8 males; mean age 7.15 years) were studied. mRNA expression of interferon (IFN)-gamma, interleukin (IL)-4, IL5, IL-13, eotaxin-1, eotaxin-2, eotaxin-3, and RANTES was studied. IFN-gamma and IL-5 expressions were significantly up-regulated in AEE patients compared with controls. Expressions of IL-4 and IL-13 were similar between AEE patients and controls. Eotaxin-1 expression was significantly up-regulated in AEE patients, whereas eotaxin-2 was up-regulated in controls. Expression of RANTES and eotaxin-3 was similar between the two groups. There was increased staining for mast cells in AEE patients compared with controls.Conclusions: Our data suggests that AEE is primarily an IL-5 selective TH2 response, with a possible TH1 component, and a differential role of eosinophilic chemoattractants. The role of mast cells in the pathogenesis of AEE needs additional study.