Chiral separation of chloroquine using heparin as a chiral selector in high-performance liquid chromatography.
Chiral separation of chloroquine using heparin as a chiral selector in high-performance liquid chromatography.
复制标题
在高效液相色谱中使用肝素作为手性选择剂进行氯喹的手性分离。
DOI：
10.1021/ac951078e
复制
发表时间：
1996
影响因子：
7.4
通讯作者：
Baldueza,M
中科院分区：
文献类型：
--
作者：
Staicup,AM;Gahm,KH;Baldueza,M
研究了市售肝素亲和柱对氯喹的手性选择性。（+）对映体首先被洗脱，这与之前报道的在毛细管区带电泳中使用肝素作为手性添加剂的结果一致。探讨了pH、离子强度和有机改性剂对对映体辨别的影响。静电和疏水相互作用的结合似乎在这种新相表现出的对映体区分中起重要作用。
The chiral selectivity of a commercially available heparin affinity column was investigated for chloroquine. The (+) enantiomer eluted first, which is consistent with results reported previously using heparin as a chiral additive in capillary zone electrophoresis. The effects of pH, ionic strength, and organic modifier on the enantiodiscrimination were explored. A combination of electrostatic and hydrophobic interactions seems to play an important role in the enantiodiscrimination exhibited by this novel phase.