Long-term environmental enrichment leads to regional increases in neurotrophin levels in rat brain
Long-term environmental enrichment leads to regional increases in neurotrophin levels in rat brain
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DOI：
10.1006/exnr.2000.7415
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发表时间：
2000-07-01
影响因子：
5.3
通讯作者：
Granholm, AC
中科院分区：
文献类型：
--
作者：
Ickes, BR;Pham, TM;Granholm, AC
许多研究表明，大脑的形态和生化指标都会随着环境的影响而发生变化。在以前的工作中，我们已经证明在丰富的环境条件下长大的大鼠比在隔离条件下长大的大鼠在空间记忆任务中表现得更好，我们还发现在基底前脑中低亲和力神经生长因子受体和高亲和力神经生长因子受体的免疫反应密度都高于隔离环境条件下的大鼠。为了确定这些变化是否也与其他脑区神经营养素水平的变化有关，我们测量了在EC或IC条件下升高的大鼠的神经营养素水平。将2个月龄和12个月龄大鼠置于不同的环境中，用Promega酶联免疫吸附分析试剂盒检测脑区神经生长因子、脑源性神经营养因子和神经营养因子-3的水平。我们发现，与年龄匹配的IC组相比，EC组的大脑皮层、海马结构、基底前脑和后脑中NGF和BDNF的水平都增加了。在EC动物的基底前脑和大脑皮层中，NT-3的表达也明显增加。这些发现表明，与隔离环境相比，在丰富的环境中，几个脑区的NGF、BDNF和NT-3蛋白水平发生了显著的变化，从而为已经发现随着环境刺激的变化而发生的行为和形态变化提供了可能的生化基础。(C)2000年学术出版社。
A number of studies have demonstrated that both morphological and biochemical indices in the brain undergo alterations in response to environmental influences. In previous work we have shown that rats raised in an enriched environmental condition (EC) perform better on a spatial memory task than rats raised in isolated conditions (IC), We have also found that EC rats have a higher density of immunoreactivity than IC rats for both low and high affinity nerve growth factor (NGF) receptors in the basal forebrain. In order to determine if these alterations were coupled with altered levels of neurotrophins in other brain regions as well, we measured neurotrophin levels in rats that were raised in EC or IC conditions. Rats were placed in the different environments at 2 months of age and 12 months later brain regions were dissected and analyzed for NGF, brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) levels using Promega ELISA kits. We found that NGF and BDNF levels were increased in the cerebral cortex, hippocampal formation, basal forebrain, and hindbrain in EC animals compared to age-matched IC animals. NT-3 was found to be increased in the basal forebrain and cerebral cortex of EC animals as well. These findings demonstrate significant alterations in NGF, BDNF, and NT-3 protein levels in several brain regions as a result of an enriched versus an isolated environment and thus provide a possible biochemical basis for behavioral and morphological alterations that have been found to occur with a shifting environmental stimulus. (C) 2000 Academic Press.