Chitosan-hyaluronic acid hydrogel for cartilage repair
Chitosan-hyaluronic acid hydrogel for cartilage repair
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DOI：
10.1016/j.ijbiomac.2017.03.142
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发表时间：
2017-11-01
影响因子：
8.2
通讯作者：
Prabha, Nair
中科院分区：
文献类型：
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作者：
Neethu, Mohan;Mohanan, P., V;Prabha, Nair
目前的研究通过两种不同的方法研究了壳聚糖-透明质酸二醛水凝胶用于体内软骨再生的潜力：单独凝胶用于软骨再生或软骨细胞和凝胶组合用于软骨修复。在新西兰白兔的膝关节中产生了临界尺寸的骨软骨缺损。将同种异体兔软骨细胞封装在水凝胶中，并将凝胶或凝胶+细胞无菌植入缺损处。植入 12 周后对再生软骨进行分析。形态学评分表明，所有动物在12周时都形成了修复组织，无论它们是假手术、接受凝胶还是以细胞作为植入物的凝胶。然而，假手术中形成的修复组织呈纤维状且不透明，而接受凝胶的修复组织具有与周围天然软骨相似的质地，并且接受凝胶+细胞的动物表现出不同的反应。 II 型胶原蛋白的组织学染色、评分分布和免疫染色显示，仅接受凝胶的动物因为植入物具有透明软骨和纤维软骨的混合物。具有细胞封装凝胶的动物有更多的纤维细胞，II 型胶原蛋白染色较弱。在封装软骨细胞存在的情况下，再生软骨的质量没有显着提高。 (C) 2017 年由 Elsevier B.V. 出版
The current study investigates the potential of chitosan-hyaluronic acid dialdehyde hydrogels for in vivo cartilage regeneration following two different approaches: Gel alone for cartilage regeneration or combination of chondrocytes and gel for cartilage repair. Critical size osteochondral defects were created in knee joints of Newzealand White rabbits. Allogenic rabbit chondrocytes were encapsulated in hydrogels and gel or gel + cells were implanted in defects aseptically. The regenerated cartilage was analyzed after 12 weeks of implantation. The morphological scoring indicates that repair tissue was formed in all the animals by 12 weeks irrespective of whether they were sham, received gel, or gel with cells as implant. However the repair tissue formed in sham appeared fibrous and opaque, where as those that received gel had texture similar to the surrounding native cartilage and animals with gel + cells showed varied response. Histology staining, score distribution and immunostaining for collagen Type II showed animals that received gel alone as the implant had a mixture of hyaline and fibro cartilage. The animals with cell encapsulated gels had more fibrous cells with weak staining for collagen type II. There was no significant enhancement in the quality of regenerated cartilage in presence of encapsulated chondrocytes. (C) 2017 Published by Elsevier B.V.