Cold acclimation and freezing tolerance - A complex interaction of light and temperature
Cold acclimation and freezing tolerance - A complex interaction of light and temperature
复制标题
DOI：
10.1104/pp.114.2.467
复制
发表时间：
1997-06-01
期刊：
影响因子：
7.4
通讯作者：
Huner, NPA
中科院分区：
文献类型：
--
作者：
Gray, GR;Chauvin, LP;Huner, NPA
通过比较五种不同温度和光照条件下的生长情况，（20 ℃和800、250和50 μ mol m（-2）s（-1）和5 ℃和250和50 μ mol m（-2）s（-1）），我们研究了光、温度和光系统II的相对还原状态对植物形态的影响，研究了冬黑麦（Secale cereale L.）的抗冻性（冻害发生的致死温度[LT 50]）、Lhcb和两个冷刺激基因（Wcs 19和Wcs 120）的转录水平以及光合调节。cv Musketeer）。我们表明，第一次，我们的知识，除了调整光合能力，非光化学猝灭能力和耐光抑制，冷诱导的转录WCS 19的积累，和紧凑的植物形态通常与冷硬化相关的光系统II的相对还原状态，而不是与生长温度或生长光照本身。相反，收购的最大LT 50，以及Lhcb和Wcs 120 mRNA的积累，似乎是依赖于生长温度和生长辐照度，但在一个独立的，添加剂的方式。结果进行了讨论，相对于可能的作用，叶绿体氧化还原平衡的调制在光合驯化折叠硬化温度和达到最大的LT 50。
By comparing growth under five different temperature and irradiance regimes (20 degrees C and 800, 250, and 50 mu mol m(-2) s(-1) and 5 degrees C and 250 and 50 mu mol m(-2) s(-1)), we have examined the effects of light, temperature, and the relative reduction state of photosystem II on plant morphology, freezing tolerance (lethal temperature at which freezing injury occurs [LT50]), transcript levels of Lhcb and two cold-stimulated genes (Wcs19 and Wcs120), and photosynthetic adjustment in winter rye (Secale cereale L. cv Musketeer). We show, for the first time to our knowledge, that in addition to adjustments in photosynthetic capacity, nonphotochemical quenching capacity and tolerance to photoinhibition, the accumulation of the cold-induced transcript Wcs19, and the compact plant morphology usually associated with cold-hardening are correlated with the relative reduction state of photosystem II rather than with growth temperature or growth irradiance per se. In contrast, the acquisition of maximal LT50, as well as Lhcb and Wcs120 mRNA accumulation, appears to be dependent on both growth temperature and growth irradiance but in an independent, additive manner. The results are discussed with respect to the possible role of the modulation of chloroplastic redox poise in photosynthetic acclimation to fold-hardening temperatures and the attainment of maximal LT50.