Proteome insights into the symbiotic relationship between a captive colony of Nasutitermes corniger and its hindgut microbiome
Proteome insights into the symbiotic relationship between a captive colony of Nasutitermes corniger and its hindgut microbiome
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DOI：
10.1038/ismej.2010.97
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发表时间：
2011-01-01
期刊：
影响因子：
11
通讯作者：
Lipton, Mary S.
中科院分区：
文献类型：
--
作者：
Burnum, Kristin E.;Callister, Stephen J.;Lipton, Mary S.
我们分析了食木白蚁 (Nasutitermes) 后肠腹中细菌群落的宏蛋白质组，并鉴定了 886 种蛋白质，其中 197 种具有已知的酶功能。使用这些酶，我们重建了完整的代谢途径，揭示了碳水化合物的运输和代谢、固氮和同化、能量产生、氨基酸合成和显着的丙酮酸铁氧还蛋白/黄素氧还蛋白氧化还原酶蛋白质冗余。我们的结果表明，与这些酶相关的活性可能比与纤维素降解相关的活性在后肠微生物群落与其白蚁宿主之间的共生关系中发挥更大的作用。 ISME 期刊 (2011) 5, 161-164； doi：10.1038/ismej.2010.97； 2010 年 7 月 8 日在线发布
We analyzed the metaproteome of the bacterial community resident in the hindgut paunch of the wood-feeding 'higher' termite (Nasutitermes) and identified 886 proteins, 197 of which have known enzymatic function. Using these enzymes, we reconstructed complete metabolic pathways revealing carbohydrate transport and metabolism, nitrogen fixation and assimilation, energy production, amino-acid synthesis and significant pyruvate ferredoxin/flavodoxin oxidoreductase protein redundancy. Our results suggest that the activity associated with these enzymes may have more of a role in the symbiotic relationship between the hindgut microbial community and its termite host than activities related to cellulose degradation. The ISME Journal (2011) 5, 161-164; doi:10.1038/ismej.2010.97; published online 8 July 2010