Visualization of exosome-mediated miR-210 transfer from hypoxic tumor cells.
Visualization of exosome-mediated miR-210 transfer from hypoxic tumor cells.
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DOI：
10.18632/oncotarget.14247
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发表时间：
2017-02-07
期刊：
影响因子：
--
通讯作者：
Chung JK
中科院分区：
文献类型：
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作者：
Jung KO;Youn H;Lee CH;Kang KW;Chung JK
癌细胞主动释放携带特定细胞成分（如蛋白质、mRNA 和 miRNA）的外泌体，以与肿瘤微环境中的各种细胞进行通讯。我们使用 miR-210 特异性报告系统可视化外泌体介导的 miR-210 从缺氧乳腺癌细胞到邻近细胞的转移。通过体外和体内可视化，我们发现带有miR-210的外泌体被转移到肿瘤微环境中的细胞中，并且miR-210参与血管重塑相关基因（例如Ephrin A3和PTP1B）的表达，以促进血管生成。这些结果表明细胞成分，例如来自缺氧癌细胞的 miRNA，通过外泌体扩散到肿瘤微环境中的邻近癌细胞并影响肿瘤进展。
Cancer cells actively release exosomes carrying specific cellular components, such as proteins, mRNA, and miRNA, to communicate with various cells in the tumor microenvironment. We visualized exosome-mediated transfer of miR-210 from hypoxic breast cancer cells to neighboring cells using a miR-210 specific reporter system. By in vitro and in vivo visualization, we found that exosomes with miR-210 were transferred to cells in the tumor microenvironment and that miR-210 was involved in expression of vascular remodeling related genes, such as Ephrin A3 and PTP1B, to promote angiogenesis. These results indicate that cellular components, such as miRNAs from hypoxic cancer cells, spread to adjacent cancer cells in the tumor microenvironment via exosomes and influence tumor progression.