Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using -Cyclodextrin
Extraction of Epigallocatechin Gallate and Epicatechin Gallate from Tea Leaves Using -Cyclodextrin
复制标题
用环糊精从茶叶中提取表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯
DOI：
10.1111/1750-3841.13622
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发表时间：
2017-02-01
影响因子：
3.9
通讯作者：
Wang, Zhouli
中科院分区：
文献类型：
--
作者：
Cui, Lu;Liu, Yuxuan;Wang, Zhouli
使用有机溶剂从植物中提取酚类化合物可能导致环境污染并引起人体健康问题。用绿色萃取剂代替有机萃取剂而不影响萃取率是目前亟待解决的问题之一。本研究的目的是评估的能力-环糊精（CD）回收茶叶中的酚类化合物。使用乙醇/水混合物得到的提取物呈现最高的总酚含量，其次是使用-CD溶液和水得到的那些。提取物的HPLC分析表明，加入-CD的提取剂对表没食子儿茶素没食子酸酯（EGCG）和表儿茶素没食子酸酯（ECG）的提取有选择性的影响。采用15 g/L-CD提取EGCG和ECG的得率高于用水和50%乙醇提取。分子对接结果表明，表没食子儿茶素没食子儿茶素、表儿茶素和没食子儿茶素更倾向于与-CD相互作用。-CD浓度，温度，研究了时间对茶叶中EGCG和ECG提取的影响，（118.7 mg/g）和ECG当用25 g/L的CD水溶液在60 ℃下提取60 min时，获得了54.6 mg/g的总黄酮。CD可用作有机溶剂的替代品，从茶叶中回收EGCG和ECG。实际应用茶儿茶素因其健康益处和功能性而受到广泛关注，并已广泛用于药物，营养品和化妆品。儿茶素的提取是其进一步使用的第一步。通常，有机溶剂用于从植物中回收酚类化合物。然而，有机溶剂的使用可能导致环境污染并引起人的健康问题。本研究证明了-CD具有替代有机溶剂从茶叶中提取EGCG和ECG的潜力。
Use of organic solvents to extract phenolic compounds from plants may result in environmental pollution and cause health problems in persons. Replacing organic extraction solvents by green extracting agents without affecting the extraction yield is one of the most pressing problems to be solved. The aim of this study is to evaluate the capacity of -cyclodextrin (-CD) to recover phenolic compounds from tea leaves. The extract obtained using the ethanol/water mixture presented the highest total phenolic content, followed by those obtained using -CD solution and water. HPLC analysis of the extracts showed that the addition of -CD to the extracting agent had a selective effect on the extraction of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). The extraction yield of EGCG and ECG using 15 g/L -CD were higher than that obtained using water and 50% ethanol. Molecular docking results indicated that the molecules of EGCG and ECG were more inclined to interact with -CD than epigallocatechin, epicatechin, and gallocatechin. The impact of -CD concentration, temperature, and time on EGCG and ECG extraction from tea leaves was investigated and the maximum amount of EGCG (118.7 mg/g) and ECG (54.6 mg/g) were achieved when extracted with 25 g/L aqueous -CD solution at 60 degrees C for 60 min. The present study indicates that aqueous -CD can be used as an alternative to organic solvents to recover EGCG and ECG from tea leaves.Practical Application Tea catechins have recently received much attention due to their health benefits and functionality and have been widely used in pharmaceuticals, nutraceuticals, and cosmetics. The extraction of catechins is the 1st step for their further use. Generally, organic solvents are used to recover phenolic compounds from plants. However, use of organic solvents may result in environmental pollution and cause health problems in persons. This study has proved that -CD has the potential to replace organic solvents to extract EGCG and ECG from tea leaves.