A detection method of recombinant DNA from genetically modified potato (NewLeaf Plus® potato) and detection of NewLeaf Plus® potato in snack
A detection method of recombinant DNA from genetically modified potato (NewLeaf Plus® potato) and detection of NewLeaf Plus® potato in snack
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DOI：
10.3358/shokueishi.43.24
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发表时间：
2002-02-01
期刊：
影响因子：
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通讯作者：
Toyoda, M
中科院分区：
文献类型：
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作者：
Akiyama, H;Sugimoto, K;Toyoda, M
建立了一种利用聚合酶链式反应(PCR)检测日本食品中未被批准为安全的转基因马铃薯(NewLeaf Plus(R)马铃薯；NL-P)的方法。以马铃薯蔗糖合成酶基因作为内对照。NL-P的DNA用一对识别PLR V-rep基因的引物特异性地提供了一条PCR扩增条带。此外，为了防止在加工马铃薯食品中感染PLRV的假阳性结果，我们设计了一对识别来自两种生物的序列的引物来特异性地检测加工马铃薯中的NL-P。用设计的引物对扩增出的产物是NL-P特异的。从含有0.05%NL-P的马铃薯粉样中可以检测到导入NL-P的DNA。将该方法用于25种马铃薯加工食品中NL-P的检测。在3种休闲食品中检出NL-P。
A detection method using polymerase chain reaction (PCR) was developed to detect the genetically modified (GM) potato (NewLeaf Plus(R) potato; NL-P), which has not been authorized as safe in foods in Japan. The potato sucrose synthase gene was used as an internal control. The DNA from NL-P specifically provided an amplified band using PCR with a primer pair recognizing PLR V-rep gene. In addition, to prevent false-positive results in processed potato foods infected with PLRV, we designed a primer pair recognizing sequences derived from two organisms to detect specifically NL-P in processed potato. The PCR product obtained using the designed primer pair was specific for NL-P. The DNA introduced into NL-P could be detected from potato powder samples containing 0.05% NL-P. The proposed method was applied to the detection of NL-P in 25 processed potato foods. NL-P was detected in 3 snack products.