Characteristic Dissection of Xanthomonas oryzae pv. oryzae Responsive MicroRNAs in Rice
Characteristic Dissection of Xanthomonas oryzae pv. oryzae Responsive MicroRNAs in Rice
复制标题
米黄单胞菌的特征解剖。
DOI：
10.3390/ijms21030785
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Zhai, Wenxue
中科院分区：
文献类型：
--
作者：
Jia, Yanfeng;Li, Chunrong;Zhai, Wenxue
microRNAs（miRNAs）是植物与病原菌相互作用的重要分子。虽然已有证据表明水稻miRNAs介导了对病原菌入侵的免疫应答，但miRNAs在黄单胞菌致病性强的水稻中的作用尚不清楚。攻击仍在进行中。本研究利用小分子RNA测序技术检测了Xoo株系PXO 86感染水稻亲和品种IR 24和不亲和品种IRBB 5后0、8、24 h水稻miRNAs的响应变化，并利用degradome技术检测了miRNAs靶基因。通过实时定量茎环逆转录-聚合酶链反应分析验证测序数据的真实性。生物信息学分析表明，差异表达的miRNAs可分为3个免疫相关簇，在感染过程中共出现80个调控单元，其中29个已知差异表达的miRNAs和38个切割靶点。此外，通过过量表达osa-miR 164 a、osa-miR 167 d和osa-miR 159 b，以及破坏调控单元osa-miR 164 a/OsNAC 60、osa-miR 167 d-5 p/OsWD 40 -174和osa-miR 159 b/OsMYBGA、OsLRR-RLK 2、OsMPK 20 -4，可能以可控的方式重置水稻对Xoo侵染的防御反应。这些发现为特征性miRNAs及其靶标在水稻-Xoo相互作用中的复杂作用提供了新的见解。
MicroRNAs (miRNAs) are crucial player in plant-pathogen interaction. While the evidence has demonstrated that rice miRNAs mediate immune response to pathogens invasion, the roles of miRNAs on Xanthomonas oryzae pv. oryzae (Xoo) attack remain be in place. Herein, we monitored the responsive changes of rice miRNAs at 0, 8, 24 h across Xoo strain PXO86 infection in its compatible rice variety IR24 and incompatible variety IRBB5 by small RNA sequencing, and the genes targeted by miRNAs were also detected via degradome technology. The faithfulness of sequencing data was validated through quantitative real-time stem-loop reverse transcription-polymerase chain reaction assay. Bioinformatic analysis showed that the differentially expressed miRNAs could be divided into three immunity-related clusters, and 80 regulatory units were emerged in infection process, which comprises 29 differentially expressed known miRNAs and 38 cleaved targets. Furthermore, the miRNA presumptive function of separate immunity cluster in rice-Xoo interplay was confirmed through overexpressing osa-miR164a, osa-miR167d and osa-miR159b, and the disruption of regulatory units, osa-miR164a/OsNAC60, osa-miR167d-5p/OsWD40-174 and osa-miR159b/OsMYBGA, OsLRR-RLK2, OsMPK20-4, may reset rice defense response to Xoo infestation in a controllable manner. These findings provide new insights into the complex roles of characteristic miRNAs and their targets in rice-Xoo interactions.