Accumulation and biological effects of metals in wild rats in mining areas of Zambia
Accumulation and biological effects of metals in wild rats in mining areas of Zambia
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DOI：
10.1007/s10661-012-2912-6
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发表时间：
2013-06-01
影响因子：
3
通讯作者：
Ishizuka, Mayumi
中科院分区：
文献类型：
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作者：
Nakayama, Shouta M. M.;Ikenaka, Yoshinori;Ishizuka, Mayumi
Kabwe市的铅锌矿和铜带省的铜钴矿是赞比亚的主要矿区。为了研究金属污染对野生动物的影响，在Kabwe和Chingola（铜带省）以及卢萨卡（非污染地点）捕获了野生黑鼠（Rattus rattus和Rattus tanezumi）。野生黑鼠在Kabwe积累了显着较高的浓度的铅和镉在各个器官比大鼠从卢萨卡。在钦戈拉，铜，钴，铅，镉的浓度显着较高的积累在野生黑大鼠比大鼠从卢萨卡。这些结果与土壤中重金属的积累规律相一致。从毒理学角度看，铅、镉在大鼠体内的浓度普遍较低。然而，金属硫蛋白-1（MT-1）和金属硫蛋白-2（MT-2）mRNA表达水平在野生黑大鼠从Kabwe显着高于大鼠从卢萨卡。广义线性模型（GLM）显示，Zn和Cu浓度对MT-1和MT-2 mRNA表达有正效应。这些结果表明，赞比亚矿区的野生黑鼠暴露于在其器官中积累的金属，引起生物反应，如MT mRNA诱导。GLM提示血红素氧合酶-1（HO-1）mRNA表达可作为铬暴露的一个指标。
The lead-zinc (Pb-Zn) mine in Kabwe City and the copper-cobalt (Cu-Co) mine in the Copperbelt Province are major mining areas in Zambia. To examine the effects of metal pollution on wildlife, wild black rats (Rattus rattus and Rattus tanezumi) were captured in Kabwe and Chingola (in the Copperbelt Province), and in Lusaka (a noncontaminated site). Wild black rats in Kabwe accumulated significantly higher concentrations of Pb and Cd in various organs than rats from Lusaka. In Chingola, significantly higher concentrations of Cu, Co, Pb, and Cd were accumulated in wild black rats than in rats from Lusaka. These results were in accordance with metal accumulation patterns in soil. From toxicological aspects, concentrations of Pb and Cd in rats were generally low. However, metallothionein-1 (MT-1) and metallothionein-2 (MT-2) mRNA expression levels in wild black rats from Kabwe were significantly higher than those in rats from Lusaka. A generalized linear model (GLM) showed that concentrations of Zn and Cu had positive effects on the MT-1 and MT-2 mRNA expression. These results suggest that wild black rats in Zambian mining sites were exposed to metals that accumulated in their organs, causing biological responses such as MT mRNA induction. GLM indicated that heme oxygenase-1 (HO-1) mRNA expression could be a marker for Cr exposure.