Identification of Single Amino Acid Polymorphisms in MS/MS Spectra of Peptides
Identification of Single Amino Acid Polymorphisms in MS/MS Spectra of Peptides
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DOI：
10.1134/s1607672911020098
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发表时间：
2011-04-01
影响因子：
0.8
通讯作者：
Archakov, A. I.
中科院分区：
文献类型：
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作者：
Chernobrovkin, A. L.;Mitkevich, V. A.;Archakov, A. I.
方法质谱。两个蛋白质组学实验的质谱数据以mzData格式从PRIDE数据库（www. ebi AC. uk/pride/）[10]（登录号见表）。第一个下载的数据集是指HUPO血浆蛋白质组计划。我们使用的实验中，主要的血浆蛋白质的馏分被删除的免疫亲和柱，之后的蛋白质进一步分馏使用固相吸附剂特异性含半胱氨酸的肽和N-糖肽的结果。该实验的数据包括两个质谱文件（表中的登录号8275和8277），其通过使用质谱仪裂解肽离子获得。
METHODSMass spectra. Mass-spectrometric data for two proteomic experiments were loaded in the mzData format from the PRIDE database (www. ebi. ac. uk/pride/)[10](for accession numbers, see the table). The first of the downloaded data sets refers to the HUPO Plasma Proteome Project. We used the results of an experiment in which the fraction of major blood plasma proteins was removed on an immunoaffinity column, after which the proteins were further fractionated using a solid-phase sorbent specific for cysteine-containing peptides and N-glycopeptides. Data of this experiment included two mass spectra files (accession numbers 8275 and 8277 in the table), which were obtained by fragmentation of peptide ions using an