Structure-Activity Relationship Studies of Strigolactone-Related Molecules for Branching Inhibition in Garden Pea: Molecule Design for Shoot Branching
Structure-Activity Relationship Studies of Strigolactone-Related Molecules for Branching Inhibition in Garden Pea: Molecule Design for Shoot Branching
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DOI：
10.1104/pp.112.195826
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发表时间：
2012-08-01
期刊：
影响因子：
7.4
通讯作者：
Rameau, Catherine
中科院分区：
文献类型：
--
作者：
Boyer, Francois-Didier;Germain, Alexandre de Saint;Rameau, Catherine
独脚金内酯（SL）最初以其在根际刺激寄生杂草如独脚金和列当属的种子萌发的作用而闻名，后来作为丛枝菌根真菌的宿主识别信号，最近被重新发现作为一类新的植物激素参与控制植物中的枝分枝。在此，我们报告了新的SL类似物的合成，据我们所知，SL的构效关系，其激素活性在豌豆（豌豆）的第一次研究。比较了它们对肉苁蓉种子萌发的作用。豌豆rmsl SL缺陷突变体用于SL生物测定，其基于在芽上直接施用SL后的腋芽长度。将该测定与其中使用水培通过根饲喂SL的测定以及与其中在施用SL后仅6小时在腋芽中定量BRANCHED 1（玉米TEOSINTE BRANCHED 1基因的豌豆同源物）的转录物水平的分子测定进行比较。我们已经证明，在同一分子中的迈克尔受体和甲基丁烯基序或二甲基丁烯基序的存在是必不可少的。已经确定，具有二甲基丁烯二醇作为D环的更有活性的类似物23可用于控制植物结构，而不强烈地有利于多枝商陆种子的萌发。粗体数字是指化合物的编号。
Initially known for their role in the rhizosphere in stimulating the seed germination of parasitic weeds such as the Striga and Orobanche species, and later as host recognition signals for arbuscular mycorrhizal fungi, strigolactones (SLs) were recently rediscovered as a new class of plant hormones involved in the control of shoot branching in plants. Herein, we report the synthesis of new SL analogs and, to our knowledge, the first study of SL structure-activity relationships for their hormonal activity in garden pea (Pisum sativum). Comparisons with their action for the germination of broomrape (Phelipanche ramosa) are also presented. The pea rms1 SL-deficient mutant was used in a SL bioassay based on axillary bud length after direct SL application on the bud. This assay was compared with an assay where SLs were fed via the roots using hydroponics and with a molecular assay in which transcript levels of BRANCHED1, the pea homolog of the maize TEOSINTE BRANCHED1 gene were quantified in axillary buds only 6 h after application of SLs. We have demonstrated that the presence of a Michael acceptor and a methylbutenolide or dimethylbutenolide motif in the same molecule is essential. It was established that the more active analog 23 with a dimethylbutenolide as the D-ring could be used to control the plant architecture without strongly favoring the germination of P. ramosa seeds. Bold numerals refer to numbers of compounds.