Visual Analytics for Robust Investigations of Placental Aquaporin Gene Expression in Response to Maternal SARS-CoV-2 Infection
Visual Analytics for Robust Investigations of Placental Aquaporin Gene Expression in Response to Maternal SARS-CoV-2 Infection
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DOI：
10.3390/analytics3010007
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发表时间：
2024-02
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通讯作者：
Raphael D. Isokpehi;Amos O. Abioye;Rickeisha S. Hamilton;Jasmin C. Fryer;A. Hollman;Antoinette M. Destefano;Kehinde B. Ezekiel;Tyrese L. Taylor;Shawna F. Brooks;Matilda O. Johnson;Olubukola Smile;Shirma Ramroop-Butts;Angela U. Makolo;Albert G. Hayward
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作者：
Raphael D. Isokpehi;Amos O. Abioye;Rickeisha S. Hamilton;Jasmin C. Fryer;A. Hollman;Antoinette M. Destefano;Kehinde B. Ezekiel;Tyrese L. Taylor;Shawna F. Brooks;Matilda O. Johnson;Olubukola Smile;Shirma Ramroop-Butts;Angela U. Makolo;Albert G. Hayward
人类胎盘是一个多功能的，盘状的临时胎儿器官，在怀孕期间在子宫内发育，连接母亲和胎儿。关于胎盘细胞类型基因表达的大规模数据集和记录母体感染不良妊娠结局的学术文章的可用性保证了使用计算资源来帮助从不同的数据源生成知识。使用母体严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）感染作为微生物感染的案例研究，我们构建了集成数据集并实施了可视化分析资源，以促进在流动，策展和分析方面对胎盘基因表达数据的稳健调查。可视化分析资源和相关数据集可以支持对SARS-CoV-2诱导的18，882个蛋白编码基因和至少1233个人类基因组/家族的人类胎盘表达水平的变化的更好理解。我们把这份报告的重点放在人类水通道蛋白基因家族，编码小的完整的膜蛋白，最初研究其在水跨细胞膜运输的作用。AQP 9是SARS-CoV-2阳性孕妇足月胎盘绒毛中唯一下调的水通道蛋白。先前的研究发现：（1）氧信号调节胎盘发育;（2）氧张力可以调节人胎盘中AQP 9的表达;（3）SARS-CoV-2可以破坏胎盘中携氧红细胞的形成。因此，未来的研究可以进行微生物感染诱导的变化（1）胎盘造血干细胞和祖细胞;和（2）胎盘表达的人水通道蛋白基因，特别是AQP 9。
The human placenta is a multifunctional, disc-shaped temporary fetal organ that develops in the uterus during pregnancy, connecting the mother and the fetus. The availability of large-scale datasets on the gene expression of placental cell types and scholarly articles documenting adverse pregnancy outcomes from maternal infection warrants the use of computational resources to aid in knowledge generation from disparate data sources. Using maternal Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection as a case study in microbial infection, we constructed integrated datasets and implemented visual analytics resources to facilitate robust investigations of placental gene expression data in the dimensions of flow, curation, and analytics. The visual analytics resources and associated datasets can support a greater understanding of SARS-CoV-2-induced changes to the human placental expression levels of 18,882 protein-coding genes and at least 1233 human gene groups/families. We focus this report on the human aquaporin gene family that encodes small integral membrane proteins initially studied for their roles in water transport across cell membranes. Aquaporin-9 (AQP9) was the only aquaporin downregulated in term placental villi from SARS-CoV-2-positive mothers. Previous studies have found that (1) oxygen signaling modulates placental development; (2) oxygen tension could modulate AQP9 expression in the human placenta; and (3) SARS-CoV-2 can disrupt the formation of oxygen-carrying red blood cells in the placenta. Thus, future research could be performed on microbial infection-induced changes to (1) the placental hematopoietic stem and progenitor cells; and (2) placental expression of human aquaporin genes, especially AQP9.