Phagocytosis of ultrasound contrast agent microbubbles by Kupffer cells
Phagocytosis of ultrasound contrast agent microbubbles by Kupffer cells
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DOI：
10.1016/j.ultrasmedbio.2006.08.008
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发表时间：
2007-02-01
影响因子：
2.9
通讯作者：
Iijima, Hiroko
中科院分区：
文献类型：
--
作者：
Yanagisawa, Kyosuke;Moriyasu, Fuminori;Iijima, Hiroko
静脉注射超声造影剂后肝脏实质期图像延迟超过5分钟被认为与巨噬细胞对造影剂微泡的吞噬作用有关。在这项研究中，我们检查了肝脏特异性巨噬细胞库普弗细胞是否吞噬微泡，以及它们的消除是否影响肝脏实质图像的延迟。相差显微镜观察表明库普弗细胞在体外吞噬各种造影剂。使用的造影剂中，99%的Sonazoid（TM）和Optison（TM）以及47%的Levovis（TM）被吞噬，而SonoVue（TM）只有7.3%和0%的Imavis（TM）被吞噬。通过氯化钆 (GdCl3) 消除体内库普弗细胞会导致 Sonazoid (TM) 和 Levovist (TM) 延迟的实质图像强度降低，而 SonoVue (TM) 与对照相比没有显示任何变化。我们的研究结果表明，库普弗细胞吞噬造影剂，并且它们是造成肝脏造影超声图像延迟的原因。
Delayed parenchymal phase images of the liver more than 5 min after IV injection of ultrasound contrast agents are thought to be related to the phagocytosis of contrast agent microbubbles by macrophages. In this study, we examined whether liver-specific macrophages, Kupffer cells, phagocytosed the microbubbles and whether their elimination affected the delayed parenchymal images of the liver. Phase-contrast microscope observations showed that Kupffer cells phagocytosed various contrast agents in vitro. Among the contrast agents used, 99% of Sonazoid (TM) and Optison (TM), and 47% of Levovist (TM) were phagocytosed, whereas only 7.3% of SonoVue (TM) and 0% of Imavist (TM) were phagocytosed. Elimination of Kupffer cells in vivo by gadolinium chloride (GdCl3) resulted in decreased intensity of the delayed parenchymal images with Sonazoid (TM) and Levovist (TM), while SonoVue (TM) showed no changes compared with control. Our findings suggested that Kupffer cells phagocytosed contrast agents and they were responsible for the delayed images of contrast ultrasound in the liver.