"You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions.
"You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus-Roseobacter interactions.
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DOI：
10.1002/pmic.201400562
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发表时间：
2015-10
期刊：
影响因子：
3.4
通讯作者：
Armengaud J
中科院分区：
文献类型：
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作者：
Christie-Oleza JA;Scanlan DJ;Armengaud J
大多数由光合初级生产者引入海洋的能量是以有机物的形式存在，这些有机物进而维持了从微生物到大型生物的食物网的其他部分。然而，光养生物和异养生物之间的相互作用对于维持海洋微生物群落的营养平衡至关重要，而这些微生物群落最终为更高的营养级提供食物。初级生产的有机物大部分被异养微生物再矿化，但是，由于大部分海洋溶解有机物是以生物聚合物的形式存在，且微生物膜转运系统作用的分子小于0.6 kDa，所以在微生物获取它之前必须在细胞外进行水解。作为海洋微生物群落的一个模拟，我们利用最先进的蛋白质组学技术，对从合成群落中获得的胞外蛋白质组进行了分析，这些合成群落结合了特定的玫瑰杆菌属（ Ruegeria pomeroyi DSS - 3、Roseobacter denitrificans OCh114和Dinoroseobacter shibae DFL - 12）和聚球藻属菌株（WH7803和WH8102）。这种方法确定了玫瑰杆菌分泌的水解酶的种类，打开了海洋浮游植物产生的生物聚合物的异养转化/再矿化的黑箱。除了突出有趣的胞外酶外，这种策略还使我们能够推断出生态位分化的分子基础的线索。
Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro‐organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. The primary produced organic matter is mostly remineralized by heterotrophic microorganisms but, because most of the oceanic dissolved organic matter is in the form of biopolymers, and microbial membrane transport systems operate with molecules <0.6 kDa, it must be hydrolyzed outside the cell before a microorganism can acquire it. As a simili of the marine microbiome, we analyzed, using state‐of‐the‐art proteomics, the exoproteomes obtained from synthetic communities combining specific Roseobacter (Ruegeria pomeroyi DSS‐3, Roseobacter denitrificans OCh114, and Dinoroseobacter shibae DFL‐12) and Synechococcus strains (WH7803 and WH8102). This approach identified the repertoire of hydrolytic enzymes secreted by Roseobacter, opening up the black box of heterotrophic transformation/remineralization of biopolymers generated by marine phytoplankton. As well as highlighting interesting exoenzymes this strategy also allowed us to infer clues on the molecular basis of niche partitioning.