Molecular and SNP characterization of two genome specific transcription factor genes GhMyb8 and GhMyb10 in cotton species
Molecular and SNP characterization of two genome specific transcription factor genes GhMyb8 and GhMyb10 in cotton species
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DOI：
10.1007/s10681-007-9485-4
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发表时间：
2008-01-01
期刊：
影响因子：
1.9
通讯作者：
Stelly, David M.
中科院分区：
文献类型：
--
作者：
Hsu, Chuan-Yu;An, Chuanfu;Stelly, David M.
从异源四倍体棉花（Gossypium hirsutum L. cv.）中分离到两个R2R3-Myb cdna （GhMyb8和GhMyb10）及其对应基因，并对其进行了鉴定。DES119)纤维细胞。GhMyb8和GhMyb10都表现出植物R2R3-MYB蛋白亚群4中共有的一些保守特征，包括GIDxxH基序和锌指结构域。基因组起源分析和单核苷酸多态性（SNP）分析表明，GhMyb8和ghmyb10是异源四倍体棉花（AD）基因组中的等位基因。GhMyb10来自A(2)亚基因组，而GhMyb8来自D-5亚基因组。通过基于SNP标记的缺失分析，探索了这两个基因可能的染色体位置。结果表明，两个基因片段中所选基因型的平均每核苷酸SNP率为3.75%（相当于每27个核苷酸有1个SNP），编码区和3′-UTR（3′非翻译区）的SNP率分别为0.55%和5.14%。Northern blot分析显示，GhMyb8和GhMyb10在所有被检测组织中均有表达，包括不同发育阶段的叶、花、根和纤维；而GhMyb8/10的转录水平在花和根中更为丰富。GhMyb10在转基因烟草植株中的异位表达表现为叶片毛状体细胞形状异常，提示GhMyb8和GhMyb10可能在毛状体细胞分化过程中发挥作用。由于细胞遗传学库的不完全覆盖，无法使用SNP删除方法确定两个myb基因的确切染色体位置。
Two R2R3-Myb cDNAs (GhMyb8 and GhMyb10) and their corresponding genes were isolated and characterized from allotetraploid cotton (Gossypium hirsutum L. cv. DES119) fiber cells. Both GhMyb8 and GhMyb10 exhibit some conserved features shared in subgroup 4 of plant R2R3-MYB proteins, including the GIDxxH motif and a zinc-finger domain. Both genomic origin and single nucleotide polymorphism (SNP) analyses reveal that GhMyb8 and 10 are alloallelic genes in the allotetraploid cotton (AD genome). GhMyb10 is derived from the A(2) subgenome, whereas GhMyb8 is from the D-5 subgenome. Possible chromosomal locations of these two genes were explored by SNP marker based deletion analyses. The results showed that the average rate of SNP per nucleotide among the selected genotypes in the two gene fragments was 3.75% (similar to one SNP per 27 nucleotide), and 0.55% and 5.14% in coding regions and 3'-UTR (3' untranslated regions), respectively. Northern blot analysis showed that GhMyb8 and GhMyb10 are expressed in all examined tissues, including leaves, flowers, roots, and fibers from different developmental stages; however, the transcript level of GhMyb8/10 is more abundant in flowers and roots. The ectopic expression of GhMyb10 in transgenic tobacco plants showed the abnormal cell shapes in leaf trichomes, suggesting that GhMyb8 and GhMyb10 might play a role in the process of trichome cell differentiation. The exact chromosomal location of the two myb genes couldn't be determined using the SNP deletion method due to the incomplete coverage of cytogenetic stocks.