Hypomorphic expression of Dkk1 in the doubleridge mouse:: dose dependence and compensatory interactions with Lrp6
Hypomorphic expression of Dkk1 in the doubleridge mouse:: dose dependence and compensatory interactions with Lrp6
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DOI：
10.1242/dev.01126
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发表时间：
2004-06-01
期刊：
影响因子：
4.6
通讯作者：
Meisler, MH
中科院分区：
文献类型：
--
作者：
MacDonald, BT;Adamska, M;Meisler, MH
双桥是一种转基因诱导的小鼠突变，表现为前肢轴后多指畸形。我们克隆了doubleridge转基因插入位点，并证明doubleridge在150 kb的距离内顺式作用，降低分泌的Wnt拮抗剂dickkopf 1 （Dkk1）的表达。Dkk1的双桥等位基因表达量在E7.0 ~ E7.0头中为野生型的35%
doubleridge is a transgene-induced mouse mutation displaying forelimb postaxial polysyndactyly. We have cloned the doubleridge transgene insertion site and demonstrate that doubleridge acts in cis from a distance of 150 kb to reduce the expression of dickkopf 1 (Dkk1), the secreted Wnt antagonist. Expression of Dkk1 from the doubleridge allele ranges from 35% of wild-type level in E7.0 head to