Generation of atmospheric pressure dry-and mist-plasma jets and their effects on HeLa cells
Generation of atmospheric pressure dry-and mist-plasma jets and their effects on HeLa cells
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DOI：
10.1615/plasmamed.2016016219
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
T. Sonoda;Kohei Umeda;Douyan Wang;T. Namihira;H. Akiyama
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文献类型：
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作者：
T. Sonoda;Kohei Umeda;Douyan Wang;T. Namihira;H. Akiyama
大气压等离子体射流由于其独特的性能和新颖的应用，最近受到了极大的关注。等离子体在空气中产生的NO2、HNO3、O3、OH等各种化学物质在水中迅速溶解和运输，伴随的化学刺激可以杀灭细菌。在我们之前的研究中，专注于OH和H2O2的生产，我们开发了一种使用干燥氦气与纯水雾混合产生的雾等离子体射流（MPJ），作为仅使用干燥氦气的传统方法的替代方案，称为干等离子体射流（DPJ）。本研究主要观察和比较了等离子体照射后即刻和24 h后MPJ和DPJ对细胞培养液包围的HeLa细胞的影响。此外，为了观察两种等离子体射流对HeLa细胞的影响的细节，准备了两个实验程序。一种是辐照培养基，包括细胞和观察细胞在24 h后更换为新鲜细胞培养基。另一种是辐照培养基，无细胞，有血浆，并观察细胞暴露于等离子体处理的培养基后24小时。这些实验表明，MPJ比DPJ更大地影响细胞死亡。
Atmospheric pressure plasma jets have recently received significant attention due to their unique capabilities and novel applications. Various chemical species, such as NO2, HNO3, O3, and OH, generated in air by plasma are dissolved and transported in water quickly, and accompanied chemical stimuli can inactivate bacteria. In our previous study, focusing on OH and H2O2 production, we developed a mist-plasma jet (MPJ) generated using dry helium gas mixed with pure water mist as an alternative to the traditional method using only dry helium gas, known as the dry-plasma jet (DPJ). In this study, the observation and comparison of effects of both MPJ and DPJ on HeLa cells surrounded by cell culture medium immediately after irradiation by plasma and following 24 h were focused. In addition, to observe details of effects of both plasma jets on HeLa cells, two experimental procedures were prepared. One is that of irradiated culture medium, including that with cells and observed cells replaced with fresh cell culture medium following 24 h. The other is that of irradiated culture medium, without cells, with plasma, and observed cells exposed to the plasma-treated culture medium after 24 h. These experiments revealed that MPJ more greatly influences cell death than DPJ.