Toll-like receptors and CD14 genes polymorphisms and susceptibility to asthma in Tunisian children
Toll-like receptors and CD14 genes polymorphisms and susceptibility to asthma in Tunisian children
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DOI：
10.1111/j.1399-0039.2008.01011.x
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发表时间：
2008-05-01
期刊：
影响因子：
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通讯作者：
Hamzaoui, A.
中科院分区：
文献类型：
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作者：
Lachheb, J.;Dhifallah, I. B.;Hamzaoui, A.
许多研究表明 CD14 和 Toll 样受体 (TLR) 2、4 和 9 在哮喘或特应性发病机制中的作用。为了评估 CD14 和 TLRs 基因多态性与哮喘或特应性的关联，本研究纳入了 210 名哮喘儿童、224 名对照者和 80 个家庭。使用聚合酶链式反应对六个单核苷酸多态性 TLR2 (+2408 G -> A)、TLR4 (+1196 C -> T)、TLR4 (+896 A -> G)、TLR9 (-1237 T -> C)、TLR9 (-1486 T -> C) 和 CD14 (-159 C -> T) 进行基因分型，然后进行限制性片段长度多态性分析。病例对照和家庭研究。 TLR9 基因多态性中的 -1237C 等位基因与哮喘风险增加相关[比值比 1.53，95% 置信区间 (1.03-2.27)]，尽管哮喘组和对照组之间其他四种 TLR 多态性的等位基因或基因型频率没有明显的统计学显着差异。与对照组相比，哮喘组的 CD14 -159 C 等位基因显着升高（P = 0.0006 < 0.05）。对 80 个哮喘家庭的传递不平衡测试显示，CD14 基因中的 -159 C 等位基因显着传递给受哮喘影响的后代。结论是 TLR9 和 CD14 基因多态性可能导致突尼斯儿童遗传性哮喘易感性。
Many studies have shown the implication of CD14 and toll-like receptors (TLRs) 2, 4 and 9 in the pathogenesis of asthma or atopy. To evaluate the association of CD14 and TLRs gene polymorphisms with asthma or atopy, 210 asthmatic children, 224 controls and 80 families were enrolled in this study. Six single nucleotide polymorphisms TLR2 (+2408 G -> A), TLR4 (+1196 C -> T), TLR4 (+896 A -> G), TLR9 (-1237 T -> C), TLR9 (-1486 T -> C) and CD14 (-159 C -> T) were genotyped using polymerase chain reaction followed by restriction fragment length polymorphism in the case-control and family study. The -1237C allele in TLR9 gene polymorphisms was associated with increased risk of asthma [odds ratio 1.53, 95% confidence interval (1.03-2.27)], although no statistically significant differences in allele or genotype frequencies of four other TLRs polymorphisms were evident between the asthmatic and control groups. The CD14 -159 C allele was found to be significantly higher in the asthmatic group when compared with controls (P = 0.0006 < 0.05). Transmission disequilibrium test of 80 asthmatic families showed significant transmission of the -159 C allele in the CD14 gene to asthma-affected offspring. It was concluded that TLR9 and CD14 gene polymorphisms may contribute to an inherited predisposition to asthma in Tunisian children.