Epstein‐barr virus subtype distribution in angioimmunoblastic lymphadenopathy
Epstein‐barr virus subtype distribution in angioimmunoblastic lymphadenopathy
复制标题
DOI：
10.1002/ijc.2910550509
复制
发表时间：
1993-11
影响因子：
6.4
通讯作者：
B. Borisch;Massimo Caioni;N. Hurwitz;C. Dommann-Scherrer;B. Odermatt;E. Waelti;R. Hubert Hubert-R.-Hubert-Hubert-1953014615;R. Kraft;J. Laissue
中科院分区：
文献类型：
--
作者：
B. Borisch;Massimo Caioni;N. Hurwitz;C. Dommann-Scherrer;B. Odermatt;E. Waelti;R. Hubert Hubert-R.-Hubert-Hubert-1953014615;R. Kraft;J. Laissue
对16例血管免疫母细胞性淋巴结病T细胞淋巴瘤(AILD-TCL)组织中EB病毒(EBV)亚型1和2的分布进行了研究。EBV-DNA的聚合酶链式反应(PCR)、EBV编码的小核RNA(EBER)的原位杂交(ISH)和EBV编码的潜伏膜蛋白(LMP)的免疫组织学检测均证实了EBV的相关性。PCR法和EBER-ISH法检测结果基本一致，但也有部分病例仅用其中一种方法检测出阳性。8/13例中有少量大细胞表达LMP。对12例患者进行EBV亚型分布调查。1例含有亚型2的EBV基因组，3例含有亚型1，4例同时含有两种亚型。有四个病例无法打字。这些发现表明，在AILD中，与艾滋病相关的淋巴瘤和致命性中线肉芽肿类型的淋巴瘤一样，可能发生了EBV亚型2，可能与这些患者逐渐发展的免疫功能障碍有关。
The tissues of 16 patients bearing a T‐cell lymphoma of angioimmunoblastic lymphadenopathy type (AILD‐TCL) were investigated for the distribution of Epstein‐Barr virus (EBV) subtypes 1and 2. EBV‐association had been proven in these cases by polymerase chain reaction (PCR) for EBV‐DNA, in situ hybridization (ISH) for EBV‐encoded small nuclear RNAs (EBER) and immunohistology for EBV‐encoded latent membrane protein (LMP). PCR and EBER‐ISH produced mostly identical results, but some cases were positive with only one of the 2 methods employed. LMP was detected in a few large cells of 8/13 cases. Twelve cases were investigated for the distribution of EBV subtypes. One case contained EBV genome of subtype 2, 3 cases contained subtype 1 and 4 cases contained both subtypes. Four cases could not be typed. These finclings suggest that in AILD, as in AIDS‐associated lymphomas and lymphomas of the lethal midline granuloma type, subtype 2 of EBV may occur, perhaps in relation to an immunodysfunction developing progressively in these patients.