COMPARISON OF THE DIRECTIGEN 1-2-3 GROUP-A STREP TEST WITH CULTURE FOR DETECTION OF GROUP-A BETA-HEMOLYTIC STREPTOCOCCI
COMPARISON OF THE DIRECTIGEN 1-2-3 GROUP-A STREP TEST WITH CULTURE FOR DETECTION OF GROUP-A BETA-HEMOLYTIC STREPTOCOCCI
复制标题
DOI：
10.1128/jcm.27.8.1715-1718.1989
复制
发表时间：
1989-08-01
影响因子：
9.4
通讯作者：
MITCHELL, RS
中科院分区：
文献类型：
--
作者：
HUCK, W;REED, BD;MITCHELL, RS
The Directigen 1-2-3 Group A Strep Test (DGAST; BBL Microbiology Systems, Cockeysville, Md.) was compared with conventional culture procedures on Trypticase soy agar with 5% sheep blood (BBL) and Selective Streptococcal Agar (ssA; BBL) for detection of group A beta-hemolytic streptococci (GABHS) for 1,006 patients complaining of sore throat. The DGAST was performed at five acute-care clinics according to the instructions of the manufacturer; interpretation of the cultures was done at the central microbiology laboratory. Of 924 patients with complete data, 243 (26.3%) were positive for GABHS on culture when both sheep blood agar and ssA were used. Of the patients with positive cultures, 159 were detected by the DGAST, yielding a sensitivity of 65.4%, a specificity of 84.7%, a positive predictive value of 60.5%, and a negative predictive value of 87.3%. The greater the number of colonies on culture, the greater the sensitivity of the DGAST, and the more intense the positive reaction on the DGAST, the higher the positive predictive value of the test. For the identification of GABHS, sheep blood agar was superior to ssA by 12.9% at 24 h and by 3.4% at 48 h of incubation.
