Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities
Comparison of ITS1 and ITS2 rDNA in 454 sequencing of hyperdiverse fungal communities
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DOI：
10.1016/j.funeco.2012.09.003
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发表时间：
2013-02-01
期刊：
影响因子：
2.9
通讯作者：
Schmitt, Imke
中科院分区：
文献类型：
--
作者：
Bazzicalupo, Anna L.;Balint, Miklos;Schmitt, Imke
新一代扩增子测序是真菌生态学研究的有力工具。技术发展表明，短片段高通量技术，如Illumina，将在真菌群落分析中变得越来越重要。因此，需要短（250 bp）和信息丰富的分子标识符。在这里，我们比较了ITS1和ITS2 rDNA使用的经验数据研究的高度多样化的叶片相关真菌群落。我们的研究结果表明，ITS2可能具有更多的可变性，并恢复了更多的分子多样性。我们证实了先前的一项硅研究，表明ITS1和IT52在与公共数据库进行比对时产生了一些不同的分类群落组成。然而，当在群落生态学背景下分析时，我们发现ITS1和ITS2在群落结构中显示出相似的模式。(C) 2012 Elsevier Ltd和英国真菌学学会。版权所有。
Next-generation amplicon sequencing is a powerful tool in ecological studies of fungi. Technological development suggests that short fragment high-throughput techniques, e.g. Illumina, will gain importance in fungal community analyses. Thus there is a need for short (250 bp) and informative molecular identifiers. Here we compared ITS1 vs. ITS2 rDNA using empirical data from a study of hyperdiverse leaf-associated fungal communities. Our results suggest that ITS2 may be more variable and recovers more of the molecular diversity. We confirm an earlier in silico study showing that ITS1 and IT52 yielded somewhat different taxonomic community compositions when blasted against public databases. However, we demonstrate that both ITS1 and ITS2 reveal similar patterns in community structure when analyzed in a community ecology context. (C) 2012 Elsevier Ltd and The British Mycological Society. All rights reserved.