Comparative analysis of liver transcriptomes associated with hypoxia tolerance in the gynogenetic blunt snout bream
Comparative analysis of liver transcriptomes associated with hypoxia tolerance in the gynogenetic blunt snout bream
复制标题
雌核发育钝口鳊鱼肝脏转录组与缺氧耐受性的比较分析
DOI：
10.1016/j.aquaculture.2020.735163
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发表时间：
2020-06-30
期刊：
影响因子：
4.5
通讯作者：
Liu, Shaojun
中科院分区：
文献类型：
--
作者：
Gong, Dingbin;Xu, Lihui;Liu, Shaojun
钝口鲷（Megalobrama amblycephala， BSB）是重要的经济鱼类，但对缺氧敏感。为了克服这一缺点，我们先前获得了抗缺氧能力增强的雌性钝口鲷（GBSB）。为了探究雌性生殖增强GBSB耐缺氧能力的分子机制，本研究对常氧条件下GBSB和BSB的肝脏转录组进行了比较分析，并鉴定了差异表达基因（DEGs）。京都基因与基因组百科全书（KEGG）通路分析显示，NF-kappa B信号通路（flip、nfkb2、cxcl8、il1b、tnfaip3、nfkbia、cox2、pias4、ube2i、malt1）、缺氧诱导因子-1 （HIF-1）信号通路（HIF-1 α、vegfa、pfkfb2、glut1、hk）、FOXO信号通路（pi3k、sgk1、foxo1、prmt1、pcka）和糖酵解/糖异生通路（pgm、hk、aldo、pcka、gapa、acs）中富集了许多deg。此外，血红蛋白的平均浓度在GBSB组高于BSB组（p < 0.05），可能与NF-kappa B和HIF-1信号通路有关。本研究为GBSB耐缺氧的潜在分子机制提供了重要信息，为GBSB抗缺氧能力的增强提供了进一步的证据，这对鱼类遗传育种具有重要意义。
The blunt snout bream (Megalobrama amblycephala, BSB) is important economic fish, but it is sensitive to hypoxia. To overcome this shortcoming, we previously obtained gynogenetic blunt snout bream (GBSB) with the enhanced ability to resist hypoxia. To explore the molecular mechanisms underlying the enhanced hypoxia tolerance of GBSB as a result of gynogenesis, in the present study, we conducted the comparative analyses of the liver transcriptomes of GBSB and BSB under normoxic conditions and identified differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that many DEGs were enriched in the NF-kappa B signaling pathway (flip, nfkb2, cxcl8, il1 b, tnfaip3, nfkbia, cox2, pias4, ube2i, malt1), hypoxia-inducible factor-1 (HIF-1) signaling pathway (hif-1 alpha, vegfa, pfkfb2, glut1, hk), FOXO signaling pathway (pi3k, sgk1, foxo1, prmt1, pcka), and glycolysis/gluconeogenesis pathway (pgm, hk, aldo, pcka, gapa, acs). In addition, the average concentration of hemoglobin, which might be related to the NF-kappa B and HIF-1 signaling pathways, was higher in GBSB than in BSB (p < 0.05). This study provided important information regarding the potential molecular mechanisms underlying hypoxia tolerance in the GBSB, presenting further evidence for the enhanced ability to resist hypoxia in GBSB, which is significant for fish genetic breeding.