FIREFLY LUMINESCENCE IN THE STUDY OF ENERGY TRANSFER MECHANISMS .1. SUBSTRATE AND ENZYME DETERMINATION
FIREFLY LUMINESCENCE IN THE STUDY OF ENERGY TRANSFER MECHANISMS .1. SUBSTRATE AND ENZYME DETERMINATION
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DOI：
10.1016/0003-9861(52)90070-2
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发表时间：
1952-01-01
影响因子：
3.9
通讯作者：
TOTTER, JR
中科院分区：
文献类型：
--
作者：
STREHLER, BL;TOTTER, JR
Farrand光荧光计，一个记录DC IP21光电倍增管和一个液氮温度量子计数器，其最终灵敏度为每毫升10-9克三磷酸腺苷（ATP），被用来测量各种化学剂对萤火虫发光的影响。可以使用Photinus pyralis的粗提取物和部分纯化的水提取物来测量在“电位”或实际ATP存在下使用砷酸盐缓冲液的产光量。微分detn。ATP和二磷酸腺苷（ADP）在彼此存在的情况下可以通过使用肌激酶来实现。磷酸-磷酸肌酸可以通过使用适当的转磷酸化酶和腺苷酸来测定。葡萄糖可以通过使用己糖激酶和ATP来测定，测量发光的抑制。氯离子对发光有很强的抑制作用，必须严格控制。肌激酶、己糖激酶、淀粉酶和肌酸腺苷转磷酸化酶可在小范围内测定。用这个系统。对某些生物过程的初步研究表明，该工具广泛适用于磷磷酸化能量机制的常规调查。
The Farrand photofluorometer, a recording DC IP21 photomultiplier, and a liquid-nitrogen-temp, quantum counter, whose ultimate sensitivity is of the order of 10-9 g. adenosine triphosphate (ATP) per ml., were used to measure the effect of various chemical agents on firefly luminescence. It was possible to use both the crude and partially purified water extracts of Photinus pyralis to measure light production in the presence of "potential" or actual ATP using arsenate buffer. Differential detn. of ATP and adenosine diphosphate (ADP) in the presence of each other is possible through the use of myokinase. Phos-phocreatine may be determined by the use of the appropriate transphosphorylase and adenylic acid. Glucose may be determined by the use of hexokinase and ATP, measuring the depression in luminescence. Chloride ion has a strong inhibitory effect on luminescence which must be controlled rigorously. Myokinase, hexokinase, apyrase, and creatine adenylic transphosphorylase may be determined in small concns. with this system. Preliminary examination of certain biological processes with this tool suggests its wide applicability for routine surveys of phos-phorylative energetic mechanisms.