Effect of a single lethal dose of TCDD on the levels of monoamines, their metabolites and tryptophan in discrete brain nuclei and peripheral tissues of Long-Evans rats.
Effect of a single lethal dose of TCDD on the levels of monoamines, their metabolites and tryptophan in discrete brain nuclei and peripheral tissues of Long-Evans rats.
复制标题
单次致死剂量的 TCDD 对 Long-Evans 大鼠离散脑核和外周组织中单胺及其代谢物和色氨酸水平的影响。
DOI：
10.1111/j.1600-0773.1993.tb01650.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. Tuomisto
中科院分区：
文献类型：
--
作者：
M. Unkila;J. Tuomisto;R. Pohjanvirta;E. Macdonald;L. Tuomisto;M. Koulu;J. Tuomisto
2，3，7，8-四氯二苯并-对-二恶英（TCDD）是大鼠体内最强的二恶英之一，其作用机制尚未确定。由于已知生物胺基本上参与控制体重和食物摄入量，其水平被确定在各种下丘脑和其他脑部位与选定的外周组织后，TCDD管理成年雄性Long-Evans大鼠。给大鼠一个单一的致死剂量的TCDD（1000微克/公斤，腹腔注射，在二甲基亚砜）或车辆单独，他们被斩首后1，5，25小时或8天TCDD管理。通过HPLC-EC分析样品中生物胺及其代谢产物的浓度。TCDD的管理增加色氨酸的浓度在8天后，曝光约20%，在几乎所有的核检查，与变化达到统计学意义的外侧下丘脑区和外侧和内侧丘脑核。重要的是，在成对喂养的对照动物中未观察到这种升高。尽管没有统计学显著性，但在暴露后第一天，大多数脑部位的多巴胺、5-羟色胺和/或5-羟基吲哚乙酸水平有降低5-10%的趋势。目前的研究结果反对的关键作用，儿茶酚胺介质的TCDD毒性。然而，脑色氨酸的延迟变化似乎不是继发于TCDD摄食不足。
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is one of the most potent anorexigens in rats with a yet unidentified mechanism of action. Since biogenic amines are known to essentially participate in the control of body weight and food intake, their levels were determined in various hypothalamic and other brain sites together with selected peripheral tissues after TCDD administration to adult male Long-Evans rats. Rats were given a single lethal dose of TCDD (1000 micrograms/kg intraperitoneally, in dimethylsulphoxide) or vehicle alone and they were decapitated at 1, 5, 25 hr or 8 days after TCDD administration. The samples were analyzed for concentrations of biogenic amines and their metabolites by HPLC-EC. Administration of TCDD increased the concentration of tryptophan at 8 days after exposure by about 20% in almost all nuclei examined, with the change reaching statistical significance in the lateral hypothalamic area and in lateral and medial accumbens nuclei. Importantly, this elevation was not seen in pair-fed control animals. Although not statistically significant, there was a tendency to 5-10% diminished dopamine, serotonin and/or 5-hydroxyindoleacetic acid levels in most brain sites during the first day postexposure. The present results argue against a crucial role for catecholamines as mediators of TCDD toxicity. However, the delayed changes in brain tryptophan do not appear to be secondary to TCDD hypophagia.
2,3,7,8-四氯二苯并-对二恶英 (TCDD) 的下丘脑作用位点。
影响因子：
3.8
作者：
Russell,DH;Buckley,AR;Shah,GN;Sipes,IG;Blask,DE;Benson,B
通讯作者：
Benson,B