Effect of topical azithromycin on corneal innate immune responses.
Effect of topical azithromycin on corneal innate immune responses.
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局部阿奇霉素对角膜先天免疫反应的影响。
DOI：
10.1167/iovs.10-5658
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发表时间：
2011
影响因子：
4.4
通讯作者：
Dana,Reza
中科院分区：
文献类型：
--
作者：
Sadrai,Zahra;Hajrasouliha,AmirReza;Chauhan,Sunil;Saban,DanielR;Dastjerdi,MohammadH;Dana,Reza
目的。目的：观察阿奇霉素（AZM）对小鼠角膜炎症模型的影响。方法：观察阿奇霉素对小鼠角膜炎症的影响。热灼法诱导BALB/c小鼠角膜炎症反应。流式细胞术分析不同时间点白细胞浸润情况。在设定的时间点，进行实时聚合酶链反应，量化角膜中不同炎症细胞因子转录物的表达。免疫组织化学分析角膜样品细胞间粘附分子-1 （ICAM-1）的表达。微球（VEGF-A）放置诱导角膜新生血管形成（CNV）。然后用AZM或载药局部治疗小鼠。结果：与载药组相比，AZM组角膜浸润明显减少。AZM还能显著降低角膜组织中白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNF-α)和ICAM-1信使RNA的表达水平。结论：炎症损伤后，局部AZM可显著减少角膜内白细胞的浸润。角膜中IL-1β、TNF-α和ICAM-1表达的相关降低进一步支持了这一点，表明AZM可能对角膜炎症具有潜在的抗炎作用。
Purpose.: To determine the effect of azithromycin (AZM) in a murine model of corneal inflammation.Methods.: The effect of topical AZM was studied in murine corneal inflammation. Corneal inflammation was induced by thermal cautery in BALB/c mice. Leukocyte infiltration at different time points was analyzed by flow cytometry. At set time points, real-time polymerase chain reaction was performed to quantify the expression of different inflammatory cytokine transcript in the cornea. Corneal samples were analyzed immunohistochemically for the expression of intercellular adhesion molecule-1 (ICAM-1). Corneal neovascularization (CNV) was induced by micropellet (VEGF-A) placement. Mice were then treated topically with either AZM or vehicle. CNV was evaluated morphometrically.Results.: Eyes receiving AZM showed a significant decrease in corneal infiltration compared with the vehicle-treated group. AZM also significantly decreased messenger RNA expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and ICAM-1 in the cornea. There was no significant difference in CNV between the AZM-and vehicle-treated groups.Conclusions.: After an inflammatory insult, topical AZM significantly reduced leukocyte infiltration into the cornea. This was further supported by an associated decrease in expression of IL-1β, TNF-α, and ICAM-1 in the cornea, indicating AZM may have a potential anti-inflammatory effect on corneal inflammation.