Repeated exposure to chlorpyrifos alters the performance of adolescent male rats in animal models of depression and anxiety
Repeated exposure to chlorpyrifos alters the performance of adolescent male rats in animal models of depression and anxiety
复制标题
反复接触毒死蜱会改变抑郁和焦虑动物模型中青春期雄性大鼠的表现
DOI：
10.1016/j.neuro.2011.03.008
复制
发表时间：
2011-08-01
期刊：
影响因子：
3.4
通讯作者：
Li, Jin
中科院分区：
文献类型：
--
作者：
Chen, Wen-Qiang;Yuan, Li;Li, Jin
毒死蜱（Chlorpyrifos，CPF）是一种广谱、高效的有机磷农药，在世界范围内广泛使用。在过去的几十年里，许多研究已经评估了急性或慢性暴露于CPF对发育中的大脑的潜在神经毒性作用。尽管CPF是一种乙酰胆碱酯酶抑制剂，但其作用不仅限于胆碱能系统，而且涉及多种神经递质系统，特别是5-羟色胺（5-HT）系统，导致5-HT相关情绪行为的长期变化。在本研究中，将4周龄的青春期雄性Sprague-Dawley大鼠以10、20、40、80和160 mg/kg/天的日剂量重复暴露于CPF（s.c.，7天），然后进行一系列与肾上腺素能功能相关的情绪行为测试，以确定CPF在青春期大鼠中的作用。行为学实验结果表明，CPF在40-160 mg/kg剂量范围内显著增加小鼠高架十字迷宫实验中进入开放臂的次数和时间，在20-160 mg/kg剂量范围内显著增加Vogel冲突实验中的电击次数，在新奇性抑制实验中显著减少摄食潜伏期。有趣的是，在强迫游泳实验中，CPF在10 mg/kg的剂量下显著增加不动时间，而在160 mg/kg的剂量下显著减少不动时间。我们的数据表明，重复暴露于CPF eliminate青春期雄性大鼠的情绪行为的改变与肾上腺素能神经系统。然而，其内在机制需要进一步研究。(C)2011 Elsevier Inc. All rights reserved.
Chlorpyrifos (CPF) is a broad spectrum, highly effective organophosphorus (OP) pesticide that has been largely used worldwide. Over the past decades, numerous studies have assessed the potential neurotoxic effects of either acute or chronic exposure to CPF on developing brain. Despite being an acetylcholinersterase inhibitor, the effects of CPF are not only confined to cholinergic system, but are involved in a wide variety of neurotransmitter systems, especially the serotonin (5-HT) system, which leads to long-lasting changes in 5-HT-related emotional behaviors. In our present study, 4-week-old adolescent male Sprague-Dawley rats were repeatedly exposed to CPF at daily doses of 10, 20, 40, 80, and 160 mg/kg/day (s.c., 7 days), and then subjected to a battery of emotional behavioral tests that related to serotonergic function in order to determine CPF effects in adolescent rats. Results in behavioral tests demonstrated CPF significantly increased the entries to and time spent in the open arms in the elevated plus-maze test at the dose of 40-160 mg/kg, the number of shocks in the Vogel's conflict test at the dose of 20-160 mg/kg, and significantly decreased the latency to feed in the novelty-suppressed feeding test in both dose range. Interestingly, in the forced swimming test, at the dose of 10 mg/kg, CPF significantly increased the immobility time, whereas it significantly decreased the immobility time at the dose of 160 mg/kg. Our data suggest that repeated exposure to CPF elicits alterations of the emotional behaviors related to serotonergic nervous system in adolescent male rats. However, the underlying mechanism needs further investigations. (C) 2011 Elsevier Inc. All rights reserved.