Pretreatment with phytoestrogen-rich plant decreases breast tumor incidence and exhibits lower profile of mammary ERα and ERβ
Pretreatment with phytoestrogen-rich plant decreases breast tumor incidence and exhibits lower profile of mammary ERα and ERβ
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DOI：
10.1016/j.maturitas.2007.08.001
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发表时间：
2007-10-20
期刊：
影响因子：
4.9
通讯作者：
Picha, Porntipa
中科院分区：
文献类型：
--
作者：
Cherdshewasart, Wichai;Panriansaen, Rattana;Picha, Porntipa
目的：植物雌激素在体外对人乳腺癌细胞具有抗增殖作用。我们测试了富含植物雌激素的葛根对大鼠乳腺癌的体内诱导作用。方法：用葛根块茎粉末以0、10、100和1000 mg/kg BW/d的剂量连续4周预处理断奶雌性Spargue-Dawley大鼠。然后用单次剂量的7，12-DMBA（80 mg/kg BW）诱导乳腺肿瘤发展，随后每周检查乳腺肿瘤的大小和多样性，持续20周，最后进行尸检。结果：1000 mg/（kg BW·d）的奇异稻瘟菌块茎粉预处理可降低大鼠肿瘤的毒力。结论：苦楝对7，12-DMBA诱导的大鼠乳腺肿瘤有一定的预防作用，其机制可能与苦楝植物雌激素与ER α和/或ER α合成抑制剂的竞争性结合有关。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Objective: Phytoestrogens have been reported to exhibit antiproliferation to human breast cancer cells in vitro. We tested the phytoestrogen-rich, Pueraria mirifica against rat breast cancer induction in vivo.Methods: The weanling female Spargue-Dawley rats were pretreated with P. mirifica tuberous powder at a dosage of 0, 10, 100 and 1000 mg/kg BW/day for four consecutive weeks. Mammary tumor development was then induced with a single dose of 7,12-DMBA, 80 mg/kg BW, followed by a weekly examination for size and multiplicity of mammary tumors for 20 weeks and finally a necropsy. Mammary tissues were investigated for the virulence of tumor and also monoclonal antibody stained against ER alpha and ER beta.Results: Pretreatment of 1000 mg/(kg BW day) of P. mirifica tuberous powder resulted in decreasing of the virulence of rat tumor development. The mammary tumor tissues exhibited lower profile of ER alpha and ER beta as well as ER alpha/ER beta.Conclusion: P mirifica exhibited prevention of 7,12-DMBA-induced rat mammary tumors, with a proposed mechanism of strong competitive binding of its phytoestrogens to ER alpha and/or synthesis suppressor of ER alpha. (c) 2007 Elsevier Ireland Ltd. All rights reserved.