Differential Expression of Toll-Like Receptors in Patients With Irritable Bowel Syndrome
Differential Expression of Toll-Like Receptors in Patients With Irritable Bowel Syndrome
复制标题
DOI：
10.1038/ajg.2010.438
复制
发表时间：
2011-02-01
影响因子：
9.8
通讯作者：
Quigley, Eamonn M. M.
中科院分区：
文献类型：
--
作者：
Brint, Elizabeth K.;MacSharry, John;Quigley, Eamonn M. M.
结论：肠易激综合征（IBS）的发病机制知之甚少。一个促成因素可能是低度粘膜炎症，可能由微生物群引发。Toll样受体（TLR）是先天免疫系统的病原体识别受体家族。本研究的目的是评估潜在的参与TLRs在IBS进一步了解参与的先天免疫系统在这个复杂的disorder.METHODS：TLRs的表达进行了研究，从26例IBS患者的结肠活检标本，并与19名健康对照组相比。免疫荧光法检测TLR4、TLR7、TLR8蛋白表达，Western blot检测TLR4蛋白表达。
OBJECTIVES: The pathogenesis of irritable bowel syndrome (IBS) is poorly understood. One contributory factor may be low-grade mucosal inflammation, perhaps initiated by the microbiota. Toll-like receptors (TLRs) are a family of pathogen-recognition receptors of the innate immune system. The aim of this study was to evaluate the potential involvement of TLRs in IBS to further understand the involvement of the innate immune system in this complex disorder.METHODS: The expression of TLRs was investigated in colonic biopsy samples obtained from 26 IBS patients and compared with 19 healthy controls. Protein expression of TLR4, TLR7, and TLR8 was confirmed by immunofluorescence and alterations in the TLR4 protein were confirmed by western blot.RESULTS: Quantitative reverse transcriptase-PCR showed increased levels of TLR4 (P