GLUCOSE-UTILIZATION AND LACTATE PRODUCTION BY HELICOBACTER-PYLORI
GLUCOSE-UTILIZATION AND LACTATE PRODUCTION BY HELICOBACTER-PYLORI
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DOI：
10.1099/00221287-139-12-3023
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发表时间：
1993-12-01
期刊：
影响因子：
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通讯作者：
BURNS, BP
中科院分区：
文献类型：
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作者：
MENDZ, GL;HAZELL, SL;BURNS, BP
用放射性示踪分析、H-1和C-13核磁共振波谱研究了D-葡萄糖在幽门螺杆菌中的转运和掺入。该细菌被发现利用D-葡萄糖，这与公认的它不能分解碳水化合物的观点相反。在实验条件下，[C-14]葡萄糖的转运速率为3.24 mmolmin(-1)(g蛋白)(-1)，掺入细胞团的速率为1.06 mU·molh(-1)(g蛋白)(-1)。[C-13]葡萄糖的利用呈现出两相特征，先是较慢的初始阶段，然后是利用速度至少快一个数量级的阶段。在这两个阶段中，葡萄糖水平的表观下降速度因菌株不同而不同，并取决于收获前细菌的生长条件。葡萄糖分解代谢的主要产物为乳酸。这些发现为幽门螺杆菌的生理学提供了新的视角，并对积极寻找适合这种微生物的治疗方法具有重要意义。
The transport and incorporation of D-glucose into the human pathogen Helicobacter pylori was investigated employing radioactive tracer analysis and H-1 and C-13 nuclear magnetic resonance spectroscopy. The bacterium was found to utilize D-glucose contrary to the accepted view that it cannot catabolize carbohydrates. Under the experimental conditions employed, the rate of transport of [C-14]glucose was 3.24 mmol min(-1) (g protein)(-1), and the rate of incorporation into the cellular mass was 1.06 mu mol h(-1) (g protein)(-1). The utilization of [C-13]glucose showed biphasic characteristics with a slower initial period followed by a phase with a rate of utilization at least an order of magnitude faster. The apparent rates of decline of glucose levels during both phases varied between strains and depended on the growth conditions of the bacteria prior to harvesting. The main product of glucose catabolism was identified as lactate. These findings provide new,perspectives into the physiology of H. pylori and have implications for the active search to develop appropriate therapies for the micro-organism.