Regulation of rat bone sialoprotein gene transcription by enamel matrix derivative.
Regulation of rat bone sialoprotein gene transcription by enamel matrix derivative.
复制标题
釉质基质衍生物对大鼠骨唾液酸蛋白基因转录的调节。
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Y. Ogata
中科院分区：
文献类型：
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作者：
E. Shimizu;Y. Nakajima;N. Kato;Youhei Nakayama;R. Saito;Hiroshi Samoto;Y. Ogata
背景
BACKGROUND
Enamel matrix derivative (EMD) has recently been developed for use as a periodontal regenerative treatment. While EMD is believed to induce regeneration of periodontal tissue, the precise mechanism is not known. Bone sialoprotein (BSP), an early phenotypic marker of osteoblast differentiation, has been implicated in the nucleation of hydroxyapatite during bone formation. In this study, we examined the ability of EMD to regulate BSP gene transcription in osteoblast-like cells.
METHODS
To determine the molecular basis of the transcriptional regulation of BSP gene transcription by EMD, we conducted Northern hybridization, transient transfection analyses with chimeric constructs of the rat BSP gene promoter linked to a luciferase reporter gene, and gel mobility shift assays.
RESULTS
Using the osteoblastic cell line ROS 17/2.8, we determined that BSP mRNA levels increased approximately 2.8-fold by EMD. In transient transfection analyses, EMD (50 microg/ml, 12 hours) increased luciferase activities of pLUC4 (nt -425 to +60) and pLUC5 (nt -801 to +60), transfected into ROS 17/2.8 cells. Within the pLUC4 and 5, a homeodomain binding element (HOX) and a transforming growth factor (TGF)-beta activation element (TAE) are present. Gel mobility shift assays with radiolabeled HOX and TAE ds-oligonucleotides revealed increased binding of nuclear proteins from EMD stimulated ROS 17/2.8 cells.
CONCLUSION
These studies have, therefore, identified EMD response elements in the rat BSP gene promoter that may mediates the effects of EMD on BSP gene transcription.
影响因子：
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作者：
H. Ryoo;H. Hoffmann;T. Beumer;Baruch Frenkel;Dwight A. Towler;G. S. Stein;J. Stein;A. J. Wijnen;J. Lian
通讯作者：
H. Ryoo;H. Hoffmann;T. Beumer;Baruch Frenkel;Dwight A. Towler;G. S. Stein;J. Stein;A. J. Wijnen;J. Lian
通过转化生长因子-β 激活元件调节 α1(I) 胶原蛋白启动子。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Ritzenthaler,JD;Goldstein,RH;Fine,A;Smith,BD
通讯作者：
Smith,BD
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Hauschka,PV;Mavrakos,AE;Iafrati,MD;Doleman,SE;Klagsbrun,M
通讯作者：
Klagsbrun,M