Cryptic diversity in a Eurasian water snake (Natrix tessellata, Serpentes: Colubridae): Evidence from mitochondrial sequence data and nuclear ISSR-PCR fingerprinting
Cryptic diversity in a Eurasian water snake (Natrix tessellata, Serpentes: Colubridae): Evidence from mitochondrial sequence data and nuclear ISSR-PCR fingerprinting
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DOI：
10.1016/j.ode.2009.03.001
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发表时间：
2009-08-25
影响因子：
1.6
通讯作者：
Wink, Michael
中科院分区：
文献类型：
--
作者：
Guicking, Daniela;Joger, Ulrich;Wink, Michael
骰子蛇，Natrix tessellata (Laurenti, 1768)，由于其广泛的古北分布，是解决欧亚系统地理学问题的合适研究生物。我们分析了几乎代表整个地理范围的300多个标本的完整线粒体细胞色素b序列和核ISSR-PCR指纹图谱。根据mtDNA序列，发现了9个主要的线粒体谱系。三个最基本的谱系分别由来自伊朗、约旦-埃及和希腊的人群组成。其他谱系与来自土耳其半岛、高加索、咸海和哈萨克斯坦东部的样本有关。在克里特岛和欧洲大陆的两个血统之间发现了姐妹群关系。假设进化速率为1.35% /百万年序列分化，谱系间的p-距离为1.7-8.4%，表明种内分化可能早在500 - 600万年前中新世/上新世过渡时期。小亚细亚和咸海地区线粒体系统发育的遗传分化模式与核ISSR-PCR分析结果不一致，表明在接触区存在一些mtDNA分支的混种。讨论了骰子蛇高种内变异的分类学意义。(C) 2009年《生物系统学报》。Elsevier GmbH出版。版权所有。
The dice snake, Natrix tessellata (Laurenti, 1768), is a suitable study organism to address questions of Eurasian phylogeography due to its wide Palearctic distribution. We analysed complete mitochondrial cytochrome b sequences and nuclear ISSR-PCR fingerprints of more than 300 specimens representing nearly the entire geographic range. Nine major mitochondrial lineages were discovered based on mtDNA sequences. The three most basal lineages comprised populations from Iran, Jordan-Egypt, and Greece, respectively. Other lineages were associated with samples from the Turkish peninsula, the Caucasus, the Aral Sea, and eastern Kazakhstan. A sister-group relationship was found between two lineages from Crete and the European mainland. Assuming an evolutionary rate of 1.35% sequence divergence per million years, among-lineage p-distances of 1.7-8.4% suggest that intraspecific differentiation might date back as far as the Miocene/Pliocene transition 5-6 million years ago. The pattern of genetic differentiation in mitochondrial phylogeny with regard to Asia Minor and the region of the Aral Sea was not congruent with the results of the nuclear ISSR-PCR analyses, and suggests admixing within some mtDNA clades at contact zones. The taxonomic implications of the high intraspecific variation in the dice snake are discussed. (C) 2009 Gesellschaft fur Biologische Systematik. Published by Elsevier GmbH. All rights reserved.