Cutting edge: Long-term B cell memory in humans after smallpox vaccination
Cutting edge: Long-term B cell memory in humans after smallpox vaccination
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DOI：
10.4049/jimmunol.171.10.4969
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发表时间：
2003-11-15
影响因子：
4.4
通讯作者：
Ahmed, R
中科院分区：
文献类型：
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作者：
Crotty, S;Felgner, P;Ahmed, R
记忆B细胞是体液免疫的核心组成部分，但对它们在人类中的寿命知之甚少。天花疫苗接种后的免疫记忆（Dry Vax）是了解B细胞记忆在没有再次暴露于If Ag的情况下的寿命的有价值的基准。在这项研究中，我们证明了天花疫苗特异性记忆B细胞在免疫个体中持续>50年。病毒特异性记忆B细胞B b最初在免疫后下降，但随后达到一个类似于峰值10倍的平台期，并在免疫后以类似于总循环IgG（+）B细胞的0.1%的频率稳定维持>50年。这些持久记忆B细胞是功能性的，并且能够在再接种后产生强有力的回忆性Ab应答。此外，在疫苗接种后数十年检测到病毒特异性CD4（+）T细胞。这些数据表明，对Dry Vax疫苗的免疫记忆是持久的，可能有助于预防天花。
Memory B cells are a central component of humoral immunity, and yet little is known about their longevity in humans. Immune memory after smallpox vaccination (Dry Vax) is a valuable benchmark for understanding the longevity of B cell memory in the absence of re-exposure to If Ag. In this study, we demonstrate that smallpox vaccine specific memory B cells last for >50 years in immunized individuals. Virus-specific memory B celb initially declined postimmunization, but then reached a plateau similar to 10-fold lower than peak and were stably maintained for >50 years after vaccination at a frequency of similar to 0.1% of total circulating IgG(+) B cells. These persisting memory B cells were functional and able to mount a robust anamnestic Ab response upon revaccination. Additionally, virus-specific CD4(+) T cells were detected decades after vaccination. These data show that immunological memory to Dry Vax vaccine is long-lived and may contribute to protection against smallpox.