The proteome of maritime pine wood forming tissue
The proteome of maritime pine wood forming tissue
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DOI：
10.1002/pmic.200401197
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发表时间：
2005-09-01
期刊：
影响因子：
3.4
通讯作者：
Plomion, C
中科院分区：
文献类型：
--
作者：
Gion, JM;Lalanne, C;Plomion, C
木材是我们最重要的自然资源之一。令人惊讶的是，我们对木材形成过程的细节几乎一无所知。这项工作的目的是描述针叶树 (Pinus pinaster Ait.) 木材形成组织中表达的主要蛋白质。使用高分辨率 2-DE，线性 pH 梯度范围为 4 至 7，总共检测到 1039 个斑点。在 MS/MS 分析的 240 个点中，67.9% 被识别，16.7% 在数据库中没有同源性，15.4% 对应于蛋白质混合物。在 MALDI-MS 分析的 57 个点中，仅识别出 15.8%。 175 种已识别蛋白质中的大多数在防御（19.4%）、碳水化合物（16.6%）和氨基酸（14.9%）代谢、基因和蛋白质表达（13.1%）、细胞骨架（8%）、细胞壁生物合成（5.7%）、次级（5.1%）和初级（4%）代谢中发挥作用。总结了已识别的蛋白质、它们的假定功能以及在不同类型木材中的行为。此信息已引入 PROTICdb 数据库，可通过 http://cbibl.cbib.u-bordeaux2.fr/Protic/Protic/home/index.php 访问。最后，将平均蛋白质含量与通过从木材形成组织中提取的 mRNA 构建的 cDNA 文库中通过 EST 计数进行定量的转录本丰度进行比较。
Wood is one of our most important natural resources. Surprisingly, we know hardly anything about the details of the process of wood formation. The aim of this work was to describe the main proteins expressed in wood forming tissue of a conifer species (Pinus pinaster Ait.). Using high resolution 2-DE with linear pH gradient ranging from 4 to 7, a total of 1039 spots were detected. Out of the 240 spots analyzed by MS/MS, 67.9% were identified, 16.7% presented no homology in the databases, and 15.4% corresponded to protein mixtures. Out of the 57 spots analyzed by MALDI-MS, only 15.8% were identified. Most of the 175 identified proteins play a role in either defense (19.4%), carbohydrates (16.6%) and amino acid (14.9%) metabolisms, genes and proteins expression (13.1%), cytoskeleton (8%), cell wall biosynthesis (5.7%), secondary (5.1%) and primary (4%) metabolisms. A summary of the identified proteins, their putative functions, and behavior in different types of wood are presented. This information was introduced into the PROTICdb database and is accessible at http://cbibl.cbib.u-bordeaux2.fr/Protic/Protic/home/index.php. Finally, the average protein amount was compared with their respective transcript abundance as quantified through ESTcounting in a cDNA-library constructed with mRNA extracted from wood forming tissue.