Cytomegalovirus Infection: Mouse Model.
Cytomegalovirus Infection: Mouse Model.
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DOI：
10.1002/cpim.51
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发表时间：
2018-08
影响因子：
--
通讯作者：
Jonjić S
中科院分区：
文献类型：
--
作者：
Brizić I;Lisnić B;Brune W;Hengel H;Jonjić S
本单元描述了用小鼠巨细胞病毒（MCMV）感染新生小鼠和成年小鼠的程序。包括用于在细胞培养物中繁殖MCMV和用于从感染小鼠的唾液腺制备更具毒性形式的MCMV的方法。提供了一种空斑测定法，用于确定感染组织或病毒库的MCMV滴度。此外，还描述了用于制备用于繁殖MCMV和用于斑块测定的小鼠胚胎成纤维细胞的方法。
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay.