Effect of rhGH and rhIGF-I treatment on protein utilization in elderly women
Effect of rhGH and rhIGF-I treatment on protein utilization in elderly women
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DOI：
10.1152/ajpendo.1997.272.1.e94
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发表时间：
1997-01-01
影响因子：
5.1
通讯作者：
Hoffman, AR
中科院分区：
文献类型：
--
作者：
Butterfield, GE;Thompson, J;Hoffman, AR
为了评估重组人生长激素（rhGH）和重组人胰岛素样生长因子I （rhIGF-I）对蛋白质利用的影响，我们邀请了14名年龄66-82岁的女性参与氮平衡（n = 14）、全身蛋白质周转（n = 14）和肌肉蛋白质合成（n = 8）的研究。在为期1个月的治疗方案的前一周和最后一周，他们被随机分配到0.025 mg rhGH/kg每日一次或0.015（低），0.03（中）或0.06（高）mg/kg每日两次的rhIGF-I。处理1周后，各组氮平衡均显著升高（P < 0.05）。1个月后，rhGH组的这种效应减弱了50%，但在所有剂量的rhIGF-I治疗期间仍保持升高。通过持续注入[N-15]甘氨酸来测量蛋白质合成和分解，rhGH（分别为9%和8%）、低剂量rhIGF-I（4.5%和4%）和高剂量rhIGF-I（18%和17%）显著增加了蛋白质合成和分解。rhGH（48%）和高、中剂量rhIGF-I（分别为27%和196%）显著增加净合成。通过[1-C-13]亮氨酸掺入测量的肌肉蛋白质合成，随着rhGH（50%）和中剂量（67%）和高剂量（57%）的rhIGF-I显著增加。这些数据表明，老年妇女的全身和肌肉蛋白质合成对生长因子刺激有反应。
To assess the effect of recombinant human growth hormone (rhGH) and recombinant human insulin-like growth factor I (rhIGF-I) an protein utilization, 14 women, age 66-82 yr, were invited to participate in studies of nitrogen balance (n = 14), whole body protein turnover (n = 14), and muscle protein synthesis (n = 8). They were studied both 1 wk before and during the last week of a 1-mo regimen, to which they had been randomly assigned, of either 0.025 mg rhGH/kg once daily or rhIGF-I at 0.015 (low), 0.03 (mid), or 0.06 (high) mg/kg twice daily. Nitrogen balance increased significantly after 1 wk of treatment in all groups (P < 0.05). After 1 mo, the magnitude of this effect had diminished by 50% in the rhGH group but remained elevated throughout the treatment period with all doses of rhIGF-I. Both protein synthesis and breakdown, measured by a primed constant infusion of [N-15]glycine, were significantly increased with rhGH (9% and 8%, respectively), low-dose rhIGF-I (4.5% and 4%), and high-dose rhIGF-I (18% and 17%). Net synthesis was significantly increased with rhGH (48%) and high- and mid-dose rhIGF-I (27% and 196%, respectively). Muscle protein synthesis as measured by incorporation of [1-C-13]leucine increased significantly with rhGH (50%) and the mid (67%) and high (57%) doses of rhIGF-I. These data show that whole body and muscle protein synthesis are responsive to growth factor stimulation in elderly women.