Curcumol inhibits the viability and invasion of colorectal cancer cells via miR-30a-5p and Hippo signaling pathway.
Curcumol inhibits the viability and invasion of colorectal cancer cells via miR-30a-5p and Hippo signaling pathway.
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Curcumol 通过 miR-30a-5p 和 Hippo 信号通路抑制结直肠癌细胞的活力和侵袭。
DOI：
10.3892/ol.2021.12560
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发表时间：
2021-04
期刊：
影响因子：
2.9
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Yu D;Liu H;Qin J;Huangfu M;Guan X;Li X;Zhou L;Dou T;Liu Y;Wang L;Fu M;Wang J;Chen X
MicroRNA-30a-5p（miR-30a-5p）是一种肿瘤抑制因子，在结直肠癌组织中表达下调，与肿瘤侵袭密切相关。然而，莪术醇在CRC恶性进展中的详细调节机制仍不清楚。采用MTT法、Transwell法、划痕法、Western blotting法和逆转录-定量PCR法检测莪术醇对大肠癌细胞活力、侵袭和迁移的抑制作用，并检测miR-30a-5p和莪术醇在大肠癌细胞侵袭和Hippo信号通路中的作用。本研究揭示miR-30a-5p在人CRC组织和细胞中表达下调。结果表明，miR-30a-5p下调伴随着Hippo信号通路的失活，这被证明可以促进CRC细胞的活力、侵袭和迁移。经鉴定，阿托洛尔处理可增加miR-30a-5p表达并激活Hippo信号通路，进而抑制CRC细胞的侵袭和迁移。miR-30a-5p的过表达增强了莪术醇对CRC细胞侵袭和迁移以及Hippo信号通路的影响。此外，下调miR-30a-5p逆转了莪术醇对CRC细胞侵袭和迁移以及Hippo信号通路的影响。这些发现确定了与miR-30a-5p相关的新信号通路，并揭示了莪术醇对miR-30a-5p表达的影响。因此，莪术醇可能作为一种潜在的治疗策略，以延迟CRC进展。
MicroRNA-30a-5p (miR-30a-5p), which functions as a tumor suppressor, has been reported to be downregulated in colorectal cancer (CRC) tissues and to be associated with cancer invasion. However, the detailed regulatory mechanism of curcumol in the malignant progression of CRC remains unknown. MTT, Transwell, scratch, western blotting and reverse transcription-quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR-30a-5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. The present study revealed that miR-30a-5p expression was downregulated in human CRC tissues and cells. The results demonstrated that miR-30a-5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. Curcumol treatment was identified to increase miR-30a-5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. Overexpression of miR-30a-5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. Furthermore, downregulation of miR-30a-5p reversed the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. These findings identified novel signaling pathways associated with miR-30a-5p and revealed the effects of curcumol on miR-30a-5p expression. Therefore, curcumol may serve as a potential therapeutic strategy to delay CRC progression.
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