BACTERIAL AND FUNGAL ACTIVITIES IN SOIL - SEPARATION OF BACTERIA AND FUNGI BY A RAPID FRACTIONATED CENTRIFUGATION TECHNIQUE
BACTERIAL AND FUNGAL ACTIVITIES IN SOIL - SEPARATION OF BACTERIA AND FUNGI BY A RAPID FRACTIONATED CENTRIFUGATION TECHNIQUE
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DOI：
10.1016/0038-0717(77)90045-1
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发表时间：
1977-01-01
影响因子：
9.7
通讯作者：
GOKSOYR, J
中科院分区：
文献类型：
--
作者：
FAEGRI, A;TORSVIK, VL;GOKSOYR, J
从挪威西部和中部的3个天然土壤中，通过均质和分馏离心可以得到2个馏分，一个馏分含有50-80%的细菌，另一个馏分含有所有真菌、土壤碎屑和剩余的细菌。当分离在低温下进行不到3-4小时时，立即使用的馏分在1-2小时内具有恒定的呼吸速率。速率的总和与未分离的土壤均质液的速率一致，从而可以计算细菌和真菌呼吸之间的比率。假设匀浆中比完整土壤中高得多的呼吸作用是由于后者的抑制条件，并且这些可以通过单独的实验进行分析，该技术允许估计给定条件下原位土壤中细菌和真菌呼吸作用之间的比率。所使用的土壤在平板和显微镜下的细菌计数值之间有很大的差异。当基于平板计数时，计算出的细菌呼吸强度变得不可能高，而当使用显微镜计数值时，它们在生物学上可能的范围内。从菌丝长度和直径测量中计算真菌的呼吸强度在生物学上是可能的。
With 3 natural soils from western and central Norway it was shown that by homogenization and fractionated centrifugations 2 fractions could be obtained, 1 containing 50-80% of the bacteria and the other all the fungi, soil debris and rest of the bacteria. When the separation was carried out in the cold for less than 3-4 h, the fractions if used immediately had constant respiratory rates for 1-2 h. The sum of the rates agreed well with that of an unfractionated soil homogenate, making it possible to calculate the ratio between bacterial and fungal respiration. Assuming that the much higher respiration in a homogenate than in intact soil is due to inhibitory conditions in the latter and that these can be analyzed by separate experiments, the technique permits estimates of the ratio between bacterial and fungal respiration in in situ soil under given conditions. The soils used had very great differences between plate and microscopic count values for bacteria. When based on plate counts the calculated bacterial respiratory intensities became impossibly high, while they were within biologically possible limits when microscopic count values were used. The respiratory intensities for fungi when calculated from hyphal lengths and diameter measurements were biologically possible.