Development of photocatalytic biosensor for the evaluation of biochemical oxygen demand
Development of photocatalytic biosensor for the evaluation of biochemical oxygen demand
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DOI：
10.1016/j.bios.2004.10.005
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发表时间：
2005-07-15
影响因子：
12.6
通讯作者：
Karube, I
中科院分区：
文献类型：
--
作者：
Chee, GJ;Nomura, Y;Karube, I
采用半导体TiO2光催化生物传感器系统，研究了河流水体生化需氧量（BOD）的测定方法。在TiO2光反应器中，以6w黑-浅-蓝荧光管为光源，对样品进行光催化。用带生物膜的氧电极测量光反应器出口样品。根据样品中生化物质的浓度不同，光催化生物传感器的响应时间在5 ~ 10 min之间。在BOD为1 mg（-1）时，传感器响应比无光催化时提高了1.33倍。光催化对单宁酸和腐植酸的降解率分别为51.8%和38.4%。阿拉伯胶和直线型烷基苯磺酸盐（LAS）对传感器的降解作用不大，但对传感器的响应大于一倍。光反应器中由光催化产生的自由基由于其寿命极短而不影响传感器的响应。对于测试样品，传感器法与常规法具有较好的相关性（r = 0.983）。该生物传感器采用光催化预处理，提高了灵敏度。(c) 2004 Elsevier B.V.版权所有
The photocatalytic biosensor of flow system using semiconductor TiO2 was developed to evaluate biochemical oxygen demand (BOD) levels in river water. Photocatalysis of sample was carried out in a photoreactor with TiO2 and a 6 W black-light blue fluorescent tube as light source. Sample from a photoreactor outlet was measured by an oxygen electrode with a biofilm. The sensor response of photocatalytic biosensor was between 5 and 10 min depending on concentration of biochemical in the samples. At BOD of 1 mg(-1), the sensor response increased 1.33-fold in comparison with that without photocatalysis. The degradation of tannic acid and humic acid with photocatalysis were 51.8 and 38.4%, respectively. Gum arabic and linear alkylbenzene sulfonate (LAS) were degraded a little, but gave the responses of more than double to the sensor. Free radicals yielded by photocatalysis in a photoreactor did not affect the sensor response because their lifetime is extremely short. Fairly good correlation (r = 0.983) between the sensor method and the conventional method was obtained for test samples. This biosensor using photocatalytic pretreatment improved the sensitivity. (c) 2004 Elsevier B.V. All rights reserved.